State Key Lab of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Biomater Sci. 2019 Mar 26;7(4):1323-1334. doi: 10.1039/c8bm01198b.
Polypropylene (PP) meshes are one of the most commonly used prosthesis materials in repairing abdominal wall defects. However, their application is usually limited due to possible serious abdominal adhesions between the mesh and the viscera. Instilling PP meshes with excellent anti-adhesion characteristics is still a formidable challenge. In this work, in order to prevent intestinal adhesion to the PP mesh, an effective method was developed to prepare anti-adhesive PP meshes, which was inspired by mussel adhesive proteins. A functional monomer, namely, dopamine methacrylamide, was first synthesized. Then, it was copolymerized with poly(ethylene glycol) methacrylate on the surface of O2-plasma-treated PP (OPP) meshes to form comb-like copolymer poly[poly(ethylene glycol) methacrylate-co-dopamine methacrylamide] (PEDMA), which was simultaneously grafted in situ on the OPP mesh surface through the catechol group of PEDMA, subsequently yielding an anti-adhesive PP mesh (OPP-g-PEDMA). The properties of PEDMA and OPP-g-PEDMA meshes were characterized by NMR, GPC, TGA, FTIR, XPS, SEM, and water contact angle measurements. NIH-3T3 cells were employed to assess the cytocompatibility of OPP-g-PEDMA in vitro. Furthermore, the rat abdominal wall defect model was used to evaluate the efficacy of adhesion prevention. The results show that OPP-g-PEDMA not only possesses fantastic biocompatibility but also satisfactory anti-adhesion property involving minimal chronic inflammation, as well as lower adhesion formation rate and adhesion tenacity scores (less than 1.0). This type of OPP-g-PEDMA mesh is a promising candidate in effectively preventing peritoneal adhesion during abdominal wall defect repair.
聚丙烯（PP）网片是修复腹壁缺损最常用的假体材料之一。然而，由于网片和内脏之间可能发生严重的腹部粘连，其应用通常受到限制。为了防止肠粘连到 PP 网片上，本工作受贻贝类黏附蛋白的启发，开发了一种在 PP 网片表面制备具有优异抗粘连性能的有效方法。首先合成了一种功能单体，即多巴胺甲基丙烯酰胺。然后，它与聚乙二醇甲基丙烯酸酯共聚在 O2-等离子体处理的 PP（OPP）网片表面上，形成梳状共聚物聚[聚乙二醇甲基丙烯酸酯-co-多巴胺甲基丙烯酰胺]（PEDMA），同时通过 PEDMA 的儿茶酚基团在 OPP 网片表面原位接枝，随后在 OPP 网片表面上生成抗粘连 PP 网片（OPP-g-PEDMA）。通过 NMR、GPC、TGA、FTIR、XPS、SEM 和水接触角测量对 PEDMA 和 OPP-g-PEDMA 网片的性能进行了表征。采用 NIH-3T3 细胞评估了 OPP-g-PEDMA 的体外细胞相容性。此外，还使用大鼠腹壁缺损模型评估了预防粘连的效果。结果表明，OPP-g-PEDMA 不仅具有极好的生物相容性，而且还具有令人满意的抗粘连性能，涉及最小的慢性炎症，以及较低的粘连形成率和粘连强度评分（小于 1.0）。这种类型的 OPP-g-PEDMA 网片是一种很有前途的候选材料，可有效防止在腹壁缺损修复过程中发生腹膜粘连。
