Huang Lin, Wang Ping, Tian Min-Qi, Zhu Li-Hua, Ye Jian-Ren
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu, 210037, China.
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu, 210037, China.
Exp Parasitol. 2019 Feb;197:51-56. doi: 10.1016/j.exppara.2019.01.004. Epub 2019 Jan 11.
The pine wood nematode Bursaphelenchus xylophilus is a disastrous pathogen of pine forests in East Asia and Europe. Despite its decimating effect on pine forests, efficient and environmentally friendly methods available to control the pine wood nematode (PWN) are limited. The most abundant protein in nematode sperm, major sperm proteins (MSPs) have only been discovered in nematodes. In this study, phylogenetic analysis showed that BxMSP10 was highly conserved in the nematode and had a closer phylogenetic relationship with free-living nematodes than with plant-parasitic nematode species. BxMSP10 was specifically expressed in the seminal vesicle of male adults. dsRNA of BxMSP10 significantly decreased reproduction, egg hatching and population maintenance in B. xylophilus. These results indicated that BxMSP10 was a potential candidate for application in the control of B. xylophilus.
松材线虫（Bursaphelenchus xylophilus）是东亚和欧洲松林的一种灾难性病原菌。尽管它对松林具有毁灭性影响，但现有的高效且环保的控制松材线虫（PWN）的方法有限。线虫精子中最丰富的蛋白质——主要精子蛋白（MSPs）仅在线虫中被发现。在本研究中，系统发育分析表明，BxMSP10在线虫中高度保守，与自由生活线虫的系统发育关系比与植物寄生线虫物种更近。BxMSP10在雄性成虫的精囊中特异性表达。BxMSP10的双链RNA显著降低了松材线虫的繁殖、卵孵化和种群维持。这些结果表明，BxMSP10是用于控制松材线虫的潜在候选物。
