转录组分析玉米（Zea mays L.）自交系 B73 幼苗期对热胁迫的响应。

Transcriptomic analysis of the maize (Zea mays L.) inbred line B73 response to heat stress at the seedling stage.

机构信息

Anhui Provincial Key Lab. of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu 241000, China.

出版信息

Gene. 2019 Apr 15;692:68-78. doi: 10.1016/j.gene.2018.12.062. Epub 2019 Jan 11.

DOI:10.1016/j.gene.2018.12.062

PMID:30641208

Abstract

High temperature is a common stress, which influences the growth and reproduction of plants. Maize is one of the most important crops all over the world. However, heat stress reduces significantly the yield and quality of maize. Therefore, it is important to illuminate molecular mechanism of maize response to heat stress. To estimate genes related to heat stress, we analyzed the transcriptome of maize in response to heat stress. In this study, six cDNA libraries were constructed form total RNA isolated from leaves of maize. A total of 35,209,446 and 35,205,472 clean reads were generated from CK (Control condition) and HTP (Heat stress condition) treatments, respectively. The results showed that 1857 DEGs were identified in maize after heat stress (1029 up-regulated and 828 down-regulated). KEGG pathway enrichment analysis for DEGs indicated that protein processing in endoplasmic reticulum pathways play a central role in maize response to heat stress. In addition, in the present study, 167 putative TFs were identified, which belong to various TF families (e.g., MYB, AP2-EREBP, b-ZIP, bHLH, NAC and WRKY), and may be associated with heat stress response of maize. This research may contribute to understand the molecular mechanism of maize inbred line B73 response to heat stress, which is beneficial for developing maize cultivars to improve yield and quality.

摘要

高温是一种常见的应激源，影响植物的生长和繁殖。玉米是全世界最重要的作物之一。然而，热应激会显著降低玉米的产量和品质。因此，阐明玉米对热应激的分子机制非常重要。为了估计与热应激相关的基因，我们分析了玉米对热应激的转录组。在这项研究中，我们从玉米叶片中分离的总 RNA 构建了 6 个 cDNA 文库。来自 CK（对照条件）和 HTP（热应激条件）处理的总 RNA 分别产生了 35209446 和 35205472 条清洁reads。结果表明，玉米在热应激后鉴定出 1857 个 DEGs（1029 个上调，828 个下调）。对 DEGs 的 KEGG 途径富集分析表明，内质网途径中的蛋白加工在玉米对热应激的反应中起核心作用。此外，在本研究中，鉴定出了 167 个假定的 TF，它们属于各种 TF 家族（如 MYB、AP2-EREBP、b-ZIP、bHLH、NAC 和 WRKY），可能与玉米的热应激反应有关。这项研究有助于理解玉米自交系 B73 对热应激的分子机制，有利于培育玉米品种提高产量和品质。