Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases; Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong; Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, 250002, People's Republic of China.
Nanotechnology. 2019 Apr 19;30(16):165702. doi: 10.1088/1361-6528/aafe36. Epub 2019 Jan 14.
Uveitis is a recurrent, sight-threatening intraocular inflammatory disease and is treated with glucocorticoids in clinical practice. In the present study, methoxypoly(ethyleneglycol)-poly(dl-lactide-co-glycolic acid) (mPEG-PLGA) nanoparticles in combination with triamcinolone acetonide (TA) were fabricated using a modified double emulsification method. Further, we characterized the TA-loaded nanoparticles, and investigated the effects of TA-loaded nanoparticles on experimental autoimmune uveitis rats, including histopathological examination and the alterations in interleukin (IL)-17 and IL-10 at mRNA and protein levels in either aqueous humor or serum. As a result, the TA-loaded nanoparticles were a well-defined spherical shape with a mean particle size of 82 nm. The in vitro release profile showed that the TA-loaded nanoparticles could sustain for more than 45 days, and possessed higher anti-inflammatory effects compared to TA alone after pathological examination, resulting in decreased IL-17 and elevated IL-10 levels in both aqueous humor and serum. Based on these findings, it can be concluded that TA-loaded mPEG-PLGA nanoparticles can potentially provide a better anti-inflammatory effect in treating chronic and recurrent uveitis in clinical practice.
葡萄膜炎是一种反复发作、威胁视力的眼内炎症性疾病，在临床实践中用糖皮质激素治疗。在本研究中，采用改良的双重乳化法制备了甲氧基聚（乙二醇）-聚（dl-乳酸-co-乙醇酸）（mPEG-PLGA）纳米粒与曲安奈德（TA）的结合物。进一步，我们对载 TA 的纳米粒进行了表征，并研究了载 TA 的纳米粒对实验性自身免疫性葡萄膜炎大鼠的影响，包括组织病理学检查以及房水或血清中白细胞介素（IL）-17 和 IL-10 在 mRNA 和蛋白水平的变化。结果，载 TA 的纳米粒呈定义明确的球形，平均粒径为 82nm。体外释放曲线表明，载 TA 的纳米粒可以持续 45 天以上，并且在病理检查后与单独 TA 相比具有更高的抗炎效果，导致房水和血清中 IL-17 降低和 IL-10 升高。基于这些发现，可以得出结论，载 TA 的 mPEG-PLGA 纳米粒在治疗慢性和复发性葡萄膜炎方面可能提供更好的抗炎效果。
