一种基于量子点到氧化石墨烯纳米片的高效荧光共振能量转移体系：在用于敌敌畏灵敏检测的光致发光适体传感探针中的应用。
An efficient fluorescence resonance energy transfer system from quantum dots to graphene oxide nano sheets: Application in a photoluminescence aptasensing probe for the sensitive detection of diazinon.
机构信息
Analytical Chemistry Laboratory, Faculty of Chemistry, University of Guilan, Namjoo Street, P.O. Box 1914, Rasht, Iran.
Analytical Chemistry Laboratory, Faculty of Chemistry, University of Guilan, Namjoo Street, P.O. Box 1914, Rasht, Iran.
出版信息
Food Chem. 2019 May 15;280:115-122. doi: 10.1016/j.foodchem.2018.12.069. Epub 2018 Dec 19.
In this paper a new aptasensor with high-sensitivity, and high-specificity for detection of diazinon, was discovered, based on fluorescence resonance energy transfer (FRET) between quantum dot (QD) as a donor and graphene oxide (GO) as an acceptor. l-cysteine capped CdS QDs/DF20 aptamer bioconjugates were successfully synthesized. GO was also attached to aptamers and photoluminescence quenching was obtained through FRET. By adding target diazinon to the bioconjugates containing GO, photoluminescence recovery was detected due to detachment of GO from the aptamer as a result of the difference in affinity for the aptamer. The detection limit of the biosensor was 0.13 nM and the linearity was maintained from 1.05 to 206 nM. Other pesticides and herbicides did not contribute to photoluminescence recovery due to lack of binding affinity for the aptamers, which demonstrates the selectivity of the biosensor. The results show the applicability of the aptasensor for monitoring diazinon in environmental and agricultural samples.
本文基于量子点（QD）作为供体和氧化石墨烯（GO）作为受体之间的荧光共振能量转移（FRET），发现了一种用于检测敌百虫的高灵敏度和高特异性的新适配体传感器。成功合成了巯基乙酸（l-cysteine）封端的 CdS QD/DF20 适体生物缀合物。GO 也被连接到适体上，并通过 FRET 获得光致发光猝灭。通过将目标敌百虫添加到含有 GO 的生物缀合物中，由于 GO 从适体上的亲和力差异而从适体上脱离，检测到光致发光恢复。该生物传感器的检测限为 0.13 nM，线性范围为 1.05 至 206 nM。由于缺乏与适体的结合亲和力，其他杀虫剂和除草剂不会导致光致发光恢复，这证明了该生物传感器的选择性。结果表明，该适体传感器适用于监测环境和农业样品中的敌百虫。
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