Ji Yalu, Cheng Mengjun, Zhai Shengjie, Xi Hengyu, Cai Ruopeng, Wang Zijing, Zhang Hao, Wang Xinwu, Xue Yibing, Li Xinwei, Sun Changjiang, Feng Xin, Lei Liancheng, Ur Rahman Sadeeq, Han Wenyu, Gu Jingmin
Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
College of Veterinary Sciences & Animal Husbandry, Abdul Wali Khan University, Mardan 23200, Pakistan.
Vet Microbiol. 2019 Feb;229:72-80. doi: 10.1016/j.vetmic.2018.12.021. Epub 2018 Dec 18.
Staphylococcus aureus is one of the most important pathogens causing rabbit necrotizing pneumonia and brings huge economic losses to rabbit production. This study investigated the preventive effect of a phage on rabbit necrotizing pneumonia caused by S. aureus. S. aureus S6 was isolated from the lungs of rabbits suffering necrotizing pneumonia and identified. A novel phage named VB-SavM-JYL01 was isolated by using S. aureus S6 as a host and showed a broader host range than the phages GH15 and K. The genome of VB-SavM-JYL01 lacked bacterial virulence-, antibiotic resistance- and lysogenesis-related genes. A single intranasal administration of VB-SavM-JYL01 (3 × 10 PFU) could effectively improve the survival rate at 48 h to 90% (9/10) compared with the survival rate of 10% and 80% observed with the PBS or linezolid treatment, respectively. The bacterial count in the lungs of rabbits treated with the phage VB-SavM-JYL01 was 4.18 × 10 CFU/g at 24 h, which was significantly decreased compared to that of rabbits treated with PBS (7.38 × 10 CFU/g) or linezolid (3.12 × 10 CFU/g). The phage treatment significantly alleviated lung tissue damage. The levels of total proteins, Panton-Valentine leukocidin (PVL), alpha-toxin (Hla) and cytokines in the lungs of the rabbits treated with the phage were significantly lower than those of the rabbits treated with PBS and similar to those of the rabbits treated with linezolid. These data demonstrate the potential utility of phage as an alternative for preventing rabbit necrotizing pneumonia caused by S. aureus.
金黄色葡萄球菌是引起兔坏死性肺炎的最重要病原体之一，给养兔业带来巨大经济损失。本研究调查了一种噬菌体对金黄色葡萄球菌引起的兔坏死性肺炎的预防效果。从患有坏死性肺炎的兔肺中分离并鉴定出金黄色葡萄球菌S6。以金黄色葡萄球菌S6为宿主分离出一种新型噬菌体VB-SavM-JYL01，其宿主范围比噬菌体GH15和K更广。VB-SavM-JYL01的基因组缺乏细菌毒力、抗生素抗性和溶原性相关基因。与分别用PBS或利奈唑胺治疗时观察到的10%和80%的存活率相比，单次鼻内给予VB-SavM-JYL01（3×10⁸ PFU）可有效将48小时的存活率提高到90%（9/10）。用噬菌体VB-SavM-JYL01治疗的兔肺中细菌计数在24小时时为4.18×10⁵ CFU/g，与用PBS（7.38×10⁶ CFU/g）或利奈唑胺（3.12×10⁶ CFU/g）治疗的兔相比显著降低。噬菌体治疗显著减轻了肺组织损伤。用噬菌体治疗的兔肺中总蛋白、杀白细胞素（PVL）、α毒素（Hla）和细胞因子的水平显著低于用PBS治疗的兔，与用利奈唑胺治疗的兔相似。这些数据证明了噬菌体作为预防金黄色葡萄球菌引起的兔坏死性肺炎的替代物的潜在效用。
