Department of the Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71122 Foggia, Italy.
Department of the Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71122 Foggia, Italy.
Ultrason Sonochem. 2019 Apr;52:477-483. doi: 10.1016/j.ultsonch.2018.12.026. Epub 2018 Dec 15.
The interest towards not-dairy beverages is increasing and almond milk is widespread diffused. The main topic of this paper was a focus on Ultrasound (US) to inhibit Escherichia coli O157:H7 and Listeria monocytogenes. The variables of the treatment (power, duration, and pulse) were combined through a randomized design; the use of DoE theory (Design of Experiments) and its outputs (Pareto chart, 3D plots, desirability profiles) pointed out that the effect of the treatment relies upon the total energy distributed into the system on E. coli O157:H7, as suggested by the significance of interactions of power, pulse and time, while power was the most important factor for L. monocytogenes. A final challenge test was done by using two combinations (H-80% of power, 8 min and pulse at 6 s- for E. coli and F-80%; 2 min; 6 s- for L. monocytogenes) and storing the samples at 4 °C for 2 weeks. This experiment suggests that the treatment could exert a sub-lethal injury on the pathogens, which, combined with the storage under refrigeration, could contribute to increase the shelf life.
人们对非乳制品饮料的兴趣日益浓厚，杏仁奶也广泛普及。本文的主要研究主题是利用超声（US）来抑制大肠杆菌 O157:H7 和单核细胞增生李斯特菌。通过随机设计组合处理变量（功率、时间和脉冲）；使用 DOE 理论（实验设计）及其输出（帕累托图、3D 图、理想度曲线）指出，处理效果取决于系统中分配的总能量对大肠杆菌 O157:H7 的影响，这表明功率、脉冲和时间的相互作用具有重要意义，而功率对单核细胞增生李斯特菌是最重要的因素。通过使用两种组合（大肠杆菌的 H-80%功率，8 分钟和 6 秒脉冲-和 F-80%；2 分钟；6 秒脉冲-用于单核细胞增生李斯特菌）进行最终挑战测试，并将样品储存在 4°C 下 2 周。该实验表明，该处理方法可能对病原体造成亚致死损伤，结合冷藏储存，可能有助于延长保质期。
