Chen Di, Qian Jingya, Huo Shuhao, Wang Feng, Ma Haile, Liu Shan
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.
Institute of Food Physical Processing Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.
Foods. 2025 May 24;14(11):1871. doi: 10.3390/foods14111871.
As one of the non-thermal technologies, the pulsed magnetic field (PMF) has increasingly attracted attention for its application in food microbial inactivation. In this study, a proteomic analysis was conducted to elucidate the molecular mechanism underlying the inactivation of by a PMF. A total of 79 proteins, comprising 65 upregulated and 14 downregulated proteins, were successfully identified as differentially expressed proteins (DEPs, >1.2-fold or <0.83-fold, -value < 0.05) in exposed to a PMF at 8 T with 20 pulses. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that PMF exposure significantly impacted nutrient transport, the composition of cytoplasmic and intracellular substances, and various metabolic processes in , such as carbohydrate metabolism, amino acid metabolism, and nicotinate and nicotinamide metabolism. The disruption of cellular functions and metabolic pathways may contribute to the death of under PMF treatment. These findings provide valuable insights and serve as a reference for further investigations into the inactivation mechanisms induced by PMFs.
作为非热技术之一，脉冲磁场（PMF）在食品微生物灭活中的应用越来越受到关注。在本研究中，进行了蛋白质组学分析，以阐明PMF使[微生物名称未给出]失活的分子机制。总共79种蛋白质，包括65种上调蛋白和14种下调蛋白，被成功鉴定为在暴露于8 T、20个脉冲的PMF下的差异表达蛋白（DEPs，>1.2倍或<0.83倍，P值<0.05）。基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路分析表明，暴露于PMF会显著影响[微生物名称未给出]的营养物质运输、细胞质和细胞内物质的组成以及各种代谢过程，如碳水化合物代谢、氨基酸代谢和烟酸及烟酰胺代谢。细胞功能和代谢途径的破坏可能导致在PMF处理下[微生物名称未给出]的死亡。这些发现提供了有价值的见解，并为进一步研究PMF诱导的失活机制提供了参考。
