Demir Emine, Gulustan Filiz, Atsal Gorkem, Dalgic Abdullah, Catli Tolgahan, Yilmaz Osman, Aktas Safiye, Olgun Leven
Clinic of Otorhinolaryngology Head and Neck Surgery, Ağrı State Hospital, Ağrı Turkey.
Clinic of Otorhinolaryngology Head and Neck Surgery, Bakırköy Dr. Sadi Konuk Training and Research Hospital, İstanbul, Turkey.
J Int Adv Otol. 2018 Dec;14(3):432-436. doi: 10.5152/iao.2018.3639.
The purpose of our study was to evaluate whether hydrogen peroxide and silver composition (H202-Ag) used in pool water disinfectant is ototoxic to individuals with tympanic membrane perforation.
The tympanic membranes of both ears of 14 Wistar-type albino female rats were perforated. Since topical application was performed, the right and left ears were categorized as two subgroups (a: right ear, b: left ear). Baseline auditory brainstem response (ABR) was measured. The groups were classified according to topical applications performed as Ia (30 mg/L H202-Ag), Ib (saline), IIa (70 mg/L H202-Ag), and IIb (saline). The topical applications were performed for 30 min/day for 10 days. The ABR was measured 24 hours after the last application, and the animals were sacrificed. Bilateral temporal bones were examined using light microscopy.
An apparent rise in the hearing thresholds of the groups Ia and Ib was not observed. However, there was an apparent rise in the hearing thresholds of the group IIa, which supports ototoxicity. According to histopathology results, there weren't any pathological findings in groups Ia and Ib and did not display special features, but a neurotoxic effect was observed in group II.
Our study shows that the H202-Ag used in pool water disinfection can have ototoxic and neurotoxic effects, particularly at high concentrations.
我们研究的目的是评估泳池水消毒剂中使用的过氧化氢和银成分（H202-Ag）对鼓膜穿孔个体是否具有耳毒性。
对14只Wistar型白化雌性大鼠的双耳鼓膜进行穿孔。由于采用局部应用，将右耳和左耳分为两个亚组（a：右耳，b：左耳）。测量基线听性脑干反应（ABR）。根据局部应用情况将各组分为Ia（30 mg/L H202-Ag）、Ib（生理盐水）、IIa（70 mg/L H202-Ag）和IIb（生理盐水）。局部应用每天进行30分钟，持续10天。在最后一次应用后24小时测量ABR，并对动物实施安乐死。使用光学显微镜检查双侧颞骨。
未观察到Ia组和Ib组听力阈值有明显升高。然而，IIa组听力阈值有明显升高，这支持了耳毒性。根据组织病理学结果，Ia组和Ib组未发现任何病理表现，也未显示特殊特征，但在II组观察到神经毒性作用。
我们的研究表明，泳池水消毒中使用的H202-Ag可能具有耳毒性和神经毒性作用，尤其是在高浓度时。
