Center for Excellence in Brain Science and Intelligence Technology , Chinese Academy of Sciences , Shanghai 200031 , People's Republic of China.
Anal Chem. 2019 Feb 19;91(4):2719-2726. doi: 10.1021/acs.analchem.8b04395. Epub 2019 Jan 30.
Imaging of cholesterol and other metabolites simultaneously by ambient mass spectrometry will greatly benefit biological studies, however, it still remains challenging. Herein, by adding acid into the desorption electrospray ionization (DESI) spray solvent, we achieved simultaneous mass spectrometry imaging of cholesterol and other metabolites directly from mouse brain sections. The introduction of acid increased the signal intensity of cholesterol in mouse brain tissues by approximately 21-fold. Additionally, the present strategy provided increased signal intensities for other metabolites up to 62-fold, as well as identification of seven more metabolites (23 vs 16 for acid-enhanced DESI vs DESI). Moreover, increased corelationships for alanine as well as putrescine and spermidine with cholesterol were discovered under acid-enhanced DESI. The potential of the present strategy in the fields of biological and medical research was demonstrated by investigating the level change for cholesterol, alanine, putrescine, and spermidine in Alzheimer's disease (AD) mouse brain.
通过环境质谱同时对胆固醇和其他代谢物进行成像将极大地有益于生物学研究，但目前仍然具有挑战性。在此，通过在解吸电喷雾电离 (DESI) 喷雾溶剂中添加酸，我们实现了直接从鼠脑切片中对胆固醇和其他代谢物进行同时的质谱成像。酸的引入使鼠脑组织中胆固醇的信号强度增加了约 21 倍。此外，本策略还将其他代谢物的信号强度提高了 62 倍，同时鉴定出了另外 7 种代谢物（酸增强 DESI 比 DESI 多 23 种）。此外，在酸增强 DESI 条件下，还发现了丙氨酸以及腐胺和精胺与胆固醇之间的相关性增加。通过研究阿尔茨海默病 (AD) 鼠脑中胆固醇、丙氨酸、腐胺和精胺的水平变化，证明了本策略在生物和医学研究领域的潜力。
