Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230026, China.
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, 230029, China.
Talanta. 2023 Jan 15;252:123864. doi: 10.1016/j.talanta.2022.123864. Epub 2022 Aug 28.
In this work, endogenous metabolites in mouse brain tissue were imaged by negative desorption electrospray ionization (DESI), ammonia assisted DESI (aa-DESI), DESI/post-photoionization (DESI/PI), and ammonia assisted DESI/PI (aa-DESI/PI) mass spectrometry imaging (MSI) strategies. The combined effect of ammonia additive and post-photoionization was found to play an important role in the enhancement of sensitivity and coverage for endogenous analytes under ambient conditions. Compared with DESI, aa-DESI/PI can provide increased signal intensities for metabolites up to 37.1-fold, as well as the imaging of nine more small metabolites (m/z < 350) (26 for aa-DESI/PI and 17 for DESI) in mouse brain tissue. The results of Pearson correlation analysis and KEGG pathway analysis showed that the enhanced imaging strategy of aa-DESI/PI can facilitate the study of endogenous metabolic pathways and molecular networks. Moreover, the imaging results of mouse tumor tissue demonstrated the promising application of aa-DESI/PI in tumor research.
在这项工作中，通过负解吸电喷雾电离（DESI）、氨辅助 DESI（aa-DESI）、DESI/后光离子化（DESI/PI）和氨辅助 DESI/PI（aa-DESI/PI）质谱成像（MSI）策略对小鼠脑组织中的内源性代谢物进行了成像。发现氨添加剂和后光离子化的联合作用对于在环境条件下增强内源性分析物的灵敏度和覆盖范围起着重要作用。与 DESI 相比，aa-DESI/PI 可以为代谢物提供高达 37.1 倍的信号强度，并可以在小鼠脑组织中成像 9 种更小的代谢物（m/z < 350）（aa-DESI/PI 为 26 种，DESI 为 17 种）。Pearson 相关分析和 KEGG 途径分析的结果表明，aa-DESI/PI 的增强成像策略有助于研究内源性代谢途径和分子网络。此外，对小鼠肿瘤组织的成像结果表明，aa-DESI/PI 在肿瘤研究中具有广阔的应用前景。
