Dzadzieva M F, Bystrova A N, Ian'kova V I, Konstantinova N D, Kats L N
Zh Mikrobiol Epidemiol Immunobiol. 1988 Nov(11):3-5.
The conditions suitable for the cultivation of Yersinia and the inhibition of their cell-wall synthesis have been selected with the aim of obtaining spheroplasts and protoplasts of these microorganisms. Penicillin, streptomycin and lithium chloride have proved to be not very suitable for this purpose as they induce essential changes in the structure of the cytoplasmic membrane in altered Yersinia forms. The addition of 1% of glycine (for Y. pseudotuberculosis), 1-1.5% of methionine in combination with growth stimulators has made it possible to obtain Yersinia spheroplasts and protoplasts with the intact cytoplasmic membrane, thus permitting the isolation and purification of the cytoplasmic membrane fraction.
为了获得这些微生物的原生质球和原生质体，已筛选出适合耶尔森氏菌培养及抑制其细胞壁合成的条件。事实证明，青霉素、链霉素和氯化锂不太适合此目的，因为它们会使形态改变的耶尔森氏菌的细胞质膜结构发生本质变化。添加1%的甘氨酸（针对假结核耶尔森氏菌）、1 - 1.5%的蛋氨酸并结合生长刺激剂，已能够获得具有完整细胞质膜的耶尔森氏菌原生质球和原生质体，从而可以分离和纯化细胞质膜组分。
