Rodicio M R, Manzanal M B, Hardisson C
Arch Microbiol. 1978 Aug 1;118(2):219-21. doi: 10.1007/BF00415732.
A procedure for protoplasts formation from Escherichia coli and Serratia marcescens by treatment with fosfomycin alone is described. This method gives high and low yields of stable protoplasts from E. coli and S. marcescens respectively. In the last case numerous spheroplasts were obtained. Electron micrographs of intact cells, protoplasts and spheroplasts are shown.
描述了一种仅用磷霉素处理从大肠杆菌和粘质沙雷氏菌形成原生质体的方法。该方法分别从大肠杆菌和粘质沙雷氏菌获得高产和低产的稳定原生质体。在后一种情况下,获得了大量的球状体。展示了完整细胞、原生质体和球状体的电子显微照片。