Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Bialystok, Poland.
Department of Organic Chemistry, Medical University of Bialystok, Bialystok, Poland.
Invest New Drugs. 2019 Oct;37(5):984-993. doi: 10.1007/s10637-018-0712-8. Epub 2019 Jan 15.
1,3,5-triazine is an important heterocyclic skeleton for mono, two or three 2-chloroethylamine groups. The study presented here provides novel information on cellular effects of 1,3,5-triazine with mono, two or three 2-chloroethylamine groups in glioblastoma LBC3, LN-18 and LN-229 cell lines. In our study, the most cytotoxic effect was observed in 1,3,5-triazine with three 2-chloroethylamine groups (12f compound). It has been demonstrated that 12f induce time- and dose-dependent cytotoxicity in all investigated glioma cell lines. Apart from that in glioblastoma cells, treated with 12f compound, we noticed strong induction of apoptosis. In conclusion, this research provides novel information concerning cellular effects of apoptosis in LBC3, LN-18 and LN-229 cell lines. Moreover, we suggest that 12f compound may be a candidate for further evaluation as an effective chemotherapeutic agent for human glioblastoma cells.
1,3,5-三嗪是一个重要的杂环骨架，可用于单、双或三个 2-氯乙胺基团。本研究提供了关于单、双或三个 2-氯乙胺基团的 1,3,5-三嗪在神经胶质瘤 LBC3、LN-18 和 LN-229 细胞系中的细胞效应的新信息。在我们的研究中，观察到具有三个 2-氯乙胺基团的 1,3,5-三嗪（12f 化合物）具有最具细胞毒性的作用。已经证明，12f 在所有研究的神经胶质瘤细胞系中诱导时间和剂量依赖性的细胞毒性。除了在神经胶质瘤细胞中，用 12f 化合物处理后，我们注意到强烈诱导细胞凋亡。总之，这项研究提供了关于 LBC3、LN-18 和 LN-229 细胞系中细胞凋亡的细胞效应的新信息。此外，我们建议 12f 化合物可能是作为人类神经胶质瘤细胞的有效化疗剂进一步评估的候选物。
