Borisova A V, Makarova N V
Samara State Technical University.
Vopr Pitan. 2016;85(3):120-125. doi: 10.24411/0042-8833-2016-00043. Epub 2016 Jun 23.
Spices are a traditional food ingredient containing phenolic antioxidants and other phytonutrients. 3 kinds of fresh herbs (dill, parsley, basil), 2 kinds of onions, 11 kinds of dried herbs (vanilla fruits, ground cinnamon bark, ground fruit of cardamom, ground ginger, ground turmeric root, nutmeg seeds, ground dried herbs basil, ground dried herbs marjoram, ground dried herbs of thyme, ground black pepper fruit, bay leaf) have been analyzed. Extraction of flavonoids and phenolic compounds from plants was carried out with 50% ethanol, then analyzed for phenolic compounds, flavonoids, antiradical activity by the DPPH method, reducing power by FRAP method, antioxidant activity in a system with linoleic acid. The results indicate a high potential of the dried herbs to block free radicals in the in vitro model experiments due to the presence of phenolic compounds (gallic acid, 900-1100 mg/100 g) compared with fresh herbs (300-400 mg gallic acid/100 g) . The highest antiradical activity among the studied plants has dried marjoram leaf extract (extract concentration required to bind 50% of the DPPH radical solution - 1 mg/ml). Antioxidant properties are most pronounced in the extract of parsley (inhibits oxidation of linoleic acid in the system at 74.6%). By the ability to exhibit the reducing ability against Fe ions, ground ginger extract differed significantly (68.04 mmol Fe/1 kg of raw material). Studies in vitro of antioxidant activity of herbs, spices and dried onions showed that these objects contain phenolic groups, flavonoids, in model experiments in vitro have high antiradical, antioxidant and reducing properties. This indicates a possible favorable role in human nutrition of herbs, onions and spices.
香料是一种传统的食品成分,含有酚类抗氧化剂和其他植物营养素。已对3种新鲜草本植物(莳萝、欧芹、罗勒)、2种洋葱、11种干草本植物(香草果实、桂皮粉、小豆蔻粉、姜粉、姜黄根粉、肉豆蔻籽、干罗勒叶粉、干马郁兰叶粉、干百里香叶粉、黑胡椒果粉、月桂叶)进行了分析。用50%乙醇从植物中提取黄酮类化合物和酚类化合物,然后通过DPPH法分析酚类化合物、黄酮类化合物、抗自由基活性,通过FRAP法分析还原能力,在亚油酸体系中分析抗氧化活性。结果表明,与新鲜草本植物(300 - 400mg没食子酸/100g)相比,由于存在酚类化合物(没食子酸,900 - 1100mg/100g),干草本植物在体外模型实验中具有较高的阻断自由基的潜力。在所研究的植物中,干马郁兰叶提取物具有最高的抗自由基活性(结合50% DPPH自由基溶液所需的提取物浓度 - 1mg/ml)。抗氧化特性在欧芹提取物中最为明显(在该体系中抑制亚油酸氧化的比例为74.6%)。通过对铁离子表现出还原能力的能力,姜粉提取物差异显著(68.04mmol铁/1kg原料)。对草本植物、香料和干洋葱的抗氧化活性进行的体外研究表明,这些物质含有酚类基团、黄酮类化合物,在体外模型实验中具有较高的抗自由基、抗氧化和还原特性。这表明草本植物、洋葱和香料在人类营养中可能具有有益作用。