ARC Industrial Transformation Training Centre In Additive Biomanufacturing, Queensland University of Technology (QUT), 60 Musk Avenue, Kelvin Grove, QLD 4059, Brisbane, Australia. Institute of Health and Biomedical Innovation, Centre for Regenerative Medicine, Queensland University of Technology (QUT), 60 Musk Avenue, Kelvin Grove, QLD 4059, Brisbane, Australia.
Biofabrication. 2019 Jun 12;11(3):035028. doi: 10.1088/1758-5090/aafec8.
We present a study on ternary nanocomposites consisting of medical grade poly(ε-caprolactone) (mPCL) matrix, hydroxyapatite nanopowder (nHA) and compatibilized magnesium fluoride nanoparticle (cMgF) fillers. MgF nanoparticles were compatibilized by following a design approach based on the material interfaces of natural bone. MgF-specific peptide-poly(ethylene glycol) conjugates were synthesized and used as surface modifiers for MgF nanoparticles similarly to the non-collagenous proteins (NPC) of bone which compatibilize hydroxyapatite nanocrystallites. Different compositions of mPCL/nHA/cMgF composites were blended together and processed into three dimensional (3D) scaffolds using solvent-free techniques including cryomilling and melt extrusion-based additive manufacturing. The use of two different inorganic fillers in mPCL resulted in nanocomposite materials with enhanced mechanical and biological properties. In particular, cMgF nanoparticles were found to be the primary constitent leading to the significant improvements in the mechanical properties of these composites. The scaffolds of the ternary nanocomposites provided the best in vitro performance in terms of osteogenic differentiation and stimulated mineralization. In summary, we demonstrated that the concept of bioinspired interface engineering facilitates the development of homogeneous ternary nanocomposites with increased processability in additive biomanufacturing. Additionally, the concept leads to scaffolds exhibiting enhanced mechanical and biological properties. Overall, these multicomponent nano-interfaced building blocks add a new group of advanced functional materials with tunable mechanical properties, degradation and bioactivity.
我们提出了一项关于三元纳米复合材料的研究，该复合材料由医用聚己内酯（mPCL）基质、羟基磷灰石纳米粉末（nHA）和相容化的氟化镁纳米颗粒（cMgF）填料组成。通过基于天然骨材料界面的设计方法，对 MgF 纳米颗粒进行了相容化处理。合成了 MgF 特异性肽-聚乙二醇（PEG）缀合物，并将其用作 MgF 纳米颗粒的表面改性剂，类似于骨的非胶原蛋白（NPC），可相容化羟基磷灰石纳米晶。将不同组成的 mPCL/nHA/cMgF 复合材料混合在一起，并使用无溶剂技术（包括冷冻粉碎和熔融挤出增材制造）加工成三维（3D）支架。在 mPCL 中使用两种不同的无机填料导致纳米复合材料具有增强的机械和生物性能。特别是，发现 cMgF 纳米颗粒是导致这些复合材料机械性能显著提高的主要成分。在成骨分化和刺激矿化方面，三元纳米复合材料的支架表现出最佳的体外性能。总之，我们证明了仿生界面工程的概念有助于开发具有增材生物制造加工性能的均匀三元纳米复合材料。此外，该概念还导致支架具有增强的机械和生物性能。总体而言，这些多组分纳米界面构建块增加了一组具有可调机械性能、降解和生物活性的新型高级功能材料。
