De Santis Roberto, D'Amora Ugo, Russo Teresa, Ronca Alfredo, Gloria Antonio, Ambrosio Luigi
Institute of Polymers, Composites and Biomaterials, National Research Council of Italy, V. le J.F. Kennedy 54 - Pad. 20 Mostra d'Oltremare, 80125, Naples, Italy.
Department of Chemical Science and Materials Technology, National Research Council of Italy, Piazzale Aldo Moro 7, 00185, Rome, Italy.
J Mater Sci Mater Med. 2015 Oct;26(10):250. doi: 10.1007/s10856-015-5582-4. Epub 2015 Sep 29.
Magnetic nanocomposite scaffolds based on poly(ε-caprolactone) and poly(ethylene glycol) were fabricated by 3D fibre deposition modelling (FDM) and stereolithography techniques. In addition, hybrid coaxial and bilayer magnetic scaffolds were produced by combining such techniques. The aim of the current research was to analyse some structural and functional features of 3D magnetic scaffolds obtained by the 3D fibre deposition technique and by stereolithography as well as features of multimaterial scaffolds in the form of coaxial and bilayer structures obtained by the proper integration of such methods. The compressive mechanical behaviour of these scaffolds was investigated in a wet environment at 37 °C, and the morphological features were analysed through scanning electron microscopy (SEM) and X-ray micro-computed tomography. The capability of a magnetic scaffold to absorb magnetic nanoparticles (MNPs) in water solution was also assessed. confocal laser scanning microscopy was used to assess the in vitro biological behaviour of human mesenchymal stem cells (hMSCs) seeded on 3D structures. Results showed that a wide range of mechanical properties, covering those spanning hard and soft tissues, can be obtained by 3D FDM and stereolithography techniques. 3D virtual reconstruction and SEM showed the precision with which the scaffolds were fabricated, and a good-quality interface between poly(ε-caprolactone) and poly(ethylene glycol) based scaffolds was observed for bilayer and coaxial scaffolds. Magnetised scaffolds are capable of absorbing water solution of MNPs, and a preliminary information on cell adhesion and spreading of hMSCs was obtained without the application of an external magnetic field.
基于聚（ε-己内酯）和聚（乙二醇）的磁性纳米复合支架通过三维纤维沉积建模（FDM）和立体光刻技术制备。此外，通过结合这些技术制备了混合同轴和双层磁性支架。当前研究的目的是分析通过三维纤维沉积技术和立体光刻获得的三维磁性支架的一些结构和功能特征，以及通过适当整合这些方法获得的同轴和双层结构形式的多材料支架的特征。在37°C的潮湿环境中研究了这些支架的压缩力学行为，并通过扫描电子显微镜（SEM）和X射线微计算机断层扫描分析了形态特征。还评估了磁性支架在水溶液中吸收磁性纳米颗粒（MNP）的能力。共聚焦激光扫描显微镜用于评估接种在三维结构上的人间充质干细胞（hMSC）的体外生物学行为。结果表明，通过三维FDM和立体光刻技术可以获得涵盖硬组织和软组织的广泛力学性能。三维虚拟重建和SEM显示了支架制造的精度，并且在双层和同轴支架中观察到基于聚（ε-己内酯）和聚（乙二醇）的支架之间具有良好质量的界面。磁化支架能够吸收MNP的水溶液，并且在不施加外部磁场的情况下获得了关于hMSC细胞粘附和铺展的初步信息。
