Faculty of Administrative and Agricultural Sciences, Corporación Universitaria Lasallista, Caldas 055440, Colombia.
Faculty of Agrarian Sciences, Universidad de Antioquia, Medellín 050036, Colombia.
Toxins (Basel). 2019 Jan 14;11(1):40. doi: 10.3390/toxins11010040.
Plant natural products can attenuate the myonecrosis caused by snake venom and their phospholipases A₂ (PLA₂). In this study, we evaluated the effects of two fractions (F4 and F6) from and purified catechin on the muscle damage caused by a myotoxic PLA₂ from Colombian venom (BaColPLA₂) in mice and by venom from Brazil in mouse phrenic nerve-diaphragm muscle (PND) preparations in vitro. Male mice were injected with PLA₂ (50 µg) in the absence or presence of F4, F6, and catechin, in the gastrocnemius muscle and then killed 3, 7, 14, and 28 h later for histopathological analysis of myonecrosis, leukocyte infiltration, and the presence of collagen. Fractions F4 and F6 (500 µg) and catechin (90 µg) significantly reduced the extent of necrosis at all-time intervals. These two fractions and catechin also attenuated the leukocyte infiltration on day 3, as did catechin on day 14. There was medium-to-moderate collagen deposition in all groups up to day 7, but greater deposition on days 14 and 28 in the presence of F6 and catechin. venom (100 µg/mL) caused slight (25%) muscle facilitation after 10 minutes and weak neuromuscular blockade (64% decrease in contractile activity after a 120-minute incubation). Pre-incubation of venom with F4 or F6 abolished the facilitation, whereas catechin, which was itself facilitatory, did not. All three fractions attenuated the venom-induced decrease in muscle contractions. These findings indicate that fractions and catechin from can reduce the muscle damage caused by venom and PLA₂. These fractions or their components could be useful for treating venom-induced local damage.
植物天然产物可以减轻蛇毒及其磷脂酶 A₂ (PLA₂)引起的肌肉坏死。在这项研究中，我们评估了来自 和纯化儿茶素的两个馏分 (F4 和 F6) 对由来自哥伦比亚 毒液 (BaColPLA₂) 的肌肉毒性 PLA₂在小鼠中引起的肌肉损伤以及由来自巴西的毒液在体外小鼠膈神经-膈肌 (PND) 制剂中引起的肌肉损伤的影响。雄性小鼠在胃经肌内注射 PLA₂ (50 µg)，并在存在或不存在 F4、F6 和儿茶素的情况下，然后在 3、7、14 和 28 小时后处死，用于肌坏死、白细胞浸润和胶原存在的组织病理学分析。F4 和 F6 馏分 (500 µg) 和儿茶素 (90 µg) 在所有时间间隔均显著减轻坏死程度。这两种馏分和儿茶素也在第 3 天减轻了白细胞浸润，而儿茶素在第 14 天也减轻了白细胞浸润。所有组在第 7 天之前都有中等到中度的胶原沉积，但在存在 F6 和儿茶素的情况下，在第 14 和 28 天沉积更多。100 µg/mL 的 毒液在 10 分钟后引起轻微 (25%) 肌肉兴奋，在 120 分钟孵育后引起弱神经肌肉阻滞 (64% 的收缩活性降低)。F4 或 F6 预孵育毒液可消除兴奋作用，而本身具有兴奋作用的儿茶素则不能。这三种馏分都能减弱毒液引起的肌肉收缩减少。这些发现表明来自 的馏分和儿茶素可以减轻 毒液和 PLA₂引起的肌肉损伤。这些馏分或其成分可能有助于治疗毒液引起的局部损伤。
