Shirasawa Y, Koketsu K
Jpn J Pharmacol. 1978 Feb;28(1):57-60. doi: 10.1254/jjp.28.57.
Bullfrog sympathetic ganglion cells treated with nicotine are hyperpolarized with application of 5-HT. This 5-HT hyperpolarization, however, was not observed if preparations were pretreated with d-TC before being treated with nicotine. When preparations were treated with ACh or carbamylcholine, which transiently depolarizes ganglion cells, hyperpolarization did take place. Such was also observed in the presence of Ringer's solution if preparations were pretreated with the K+-free Ringer's solution. These results suggested that ganglion cells were hyperpolarized by the action of 5-HT when the Na+-pump of these cells was accelerated by accumulation of intracellular Na+, as the result of a transient depolarization or extracellular K+ deficiency.
用尼古丁处理过的牛蛙交感神经节细胞在施加5-羟色胺（5-HT）时会发生超极化。然而，如果在用尼古丁处理之前先用d-筒箭毒碱（d-TC）预处理标本，就不会观察到这种5-HT超极化现象。当用乙酰胆碱（ACh）或氨甲酰胆碱处理标本，使神经节细胞短暂去极化时，超极化确实会发生。如果先用无钾林格氏液预处理标本，在林格氏液存在的情况下也会观察到这种现象。这些结果表明，当这些细胞的钠泵因细胞内钠的积累（这是短暂去极化或细胞外钾缺乏的结果）而加速时，神经节细胞会因5-HT的作用而发生超极化。
