Abou Mossallam Ahlam A, El Nahas Soheir M, Mahfouz Eman R, Osman Noha M
Department of Cell Biology, Genetic Engineering Division, National Research Center, Dokki, Giza, Egypt.
Department of Molecular and Computational Biology, University of Southern California, 1050 Childs Way RRI 316, Los Angeles, CA 90089, USA.
J Genet Eng Biotechnol. 2015 Jun;13(1):71-77. doi: 10.1016/j.jgeb.2014.12.007. Epub 2015 Jan 31.
River buffalo ( with a population over 135 million heads is an important livestock. Interleukin 8 (IL-8) is a member of the chemokine family and is an important chemoattractant for neutrophils associated with a wide variety of inflammatory diseases such as endometritis. Tissue samples from the mammary gland, uterus and ovary were obtained from river buffalo (Mediterranean type) with and without endometritis. Bacteriological examination showed the presence of both gram positive and negative in all buffalo with endometritis. RNA extraction and complementary DNA (cDNA) synthesis were conducted from all tissues. Specific primer for IL8 full coding regions was designed using known cDNA sequences of , Genbank accession number AY952930.1. IL-8 gene expression was investigated in buffalo tissues. Expression of IL-8 in buffalo with endometritis was found to increase significantly over buffalo without endometritis only in the uterus ( = 0.0159). PCR products from uterus tissues (target organs) of buffalo with and without endometritis, were purified and sequenced. No polymorphic sites were detected in the investigated samples. IL-8 cDNA nucleotide sequences of buffalo with and without endometritis were 100% identical (accession number JX413057). Buffalo IL8 cDNAs were compared with corresponding sequences of member of subfamily Bovinae (buffalo and cattle) and subfamily Caprinae (sheep and goat). IL-8 species specific differences were identified.
河水牛（数量超过1.35亿头）是一种重要的家畜。白细胞介素8（IL-8）是趋化因子家族的成员，是与多种炎症性疾病（如子宫内膜炎）相关的中性粒细胞的重要趋化因子。从患有和未患有子宫内膜炎的河水牛（地中海型）获取乳腺、子宫和卵巢的组织样本。细菌学检查显示，所有患有子宫内膜炎的水牛体内均存在革兰氏阳性菌和阴性菌。从所有组织中进行RNA提取和互补DNA（cDNA）合成。使用Genbank登录号AY952930.1的已知cDNA序列设计IL8全编码区的特异性引物。研究了IL-8基因在水牛组织中的表达。发现患有子宫内膜炎的水牛中IL-8的表达仅在子宫中比未患子宫内膜炎的水牛显著增加（P = 0.0159）。对患有和未患有子宫内膜炎的水牛子宫组织（靶器官）的PCR产物进行纯化和测序。在所研究的样本中未检测到多态性位点。患有和未患有子宫内膜炎的水牛的IL-8 cDNA核苷酸序列100%相同（登录号JX413057）。将水牛IL8 cDNA与牛亚科（水牛和牛）和山羊亚科（绵羊和山羊）成员的相应序列进行比较。鉴定出IL-8物种特异性差异。
