Poniewozik Monika, Parzymies Marzena, Szot Paweł, Rubinowska Katarzyna
Subdepartment of Ornamental Plants and Dendrology, Institute of Horticultural Production, Faculty of Horticulture and Landscape Architecture, University of Life Sciences in Lublin, ul. Głęboka 28, 20-612 Lublin, Poland.
Department of Botany and Plant Physiology, Faculty of Environmental Biology, University of Life Sciences in Lublin, ul. Akademicka 15, 20-950 Lublin, Poland.
Plants (Basel). 2021 Mar 19;10(3):582. doi: 10.3390/plants10030582.
To obtain healthy and good quality plants from in vitro cultivation, it is necessary to produce plantlets with well-developed rooting systems because they must undergo acclimatization, a final and a very difficult stage of micropropagation. In the present research, the effect of auxins NAA, IAA and IBA in concentrations of 0.5; 1; 2.5 and 5 mg·dm on the in vitro rooting was studied, and it was noted that 1 mg·dm of IAA or IBA enabled the obtaining of a lot of rooted and good quality plantlets. The subsequent influence of the two most advantageous auxins on the acclimatization of plantlets in different substrates (sphagnum moss, sphagnum moss + substrate for orchids, substrate for orchids, substrate for orchids + acid peat) was tested, in the means of morphological features of plants and their physiological parameters, i.e., chlorophyll fluorescence (FV, Fm, Fv/Fm), stress enzyme activity (catalase, ascorbate peroxidase), and water balance. Considering all the tested features, it might be stated that the best results were obtained when explants were rooted in vitro in the presence of 1 mg·dm of IAA and then planted ex vitro in substrate for orchids.
为了通过离体培养获得健康且品质优良的植株，有必要培育出根系发达的幼苗，因为它们必须经历驯化这一微繁殖的最后且非常困难的阶段。在本研究中，研究了浓度为0.5、1、2.5和5 mg·dm 的生长素萘乙酸（NAA）、吲哚乙酸（IAA）和吲哚丁酸（IBA）对离体生根的影响，结果发现1 mg·dm 的IAA或IBA能够培育出大量生根且品质优良的幼苗。随后测试了两种最具优势的生长素对不同基质（水苔、水苔 + 兰花基质、兰花基质、兰花基质 + 酸性泥炭）中幼苗驯化的影响，通过植物的形态特征及其生理参数进行评估，即叶绿素荧光（FV、Fm、Fv/Fm）、应激酶活性（过氧化氢酶、抗坏血酸过氧化物酶）和水分平衡。综合所有测试特征，可以得出结论，当外植体在1 mg·dm 的IAA存在下进行离体生根，然后在兰花基质中进行离体种植时，能获得最佳结果。
