Venkataiah Peddaboina, Bhanuprakash Pittampalli, Suman Kalyan Sadhu, Subhash Karampuri
Department of Microbiology, Kakatiya University, Warangal 506 009, India.
Department of Biotechnology, Kakatiya University, Warangal 506 009, India.
J Genet Eng Biotechnol. 2016 Jun;14(1):55-60. doi: 10.1016/j.jgeb.2016.02.001. Epub 2016 Mar 12.
A plant regeneration protocol via somatic embryogenesis was achieved in cotyledon and leaf explants of , when cultured on MS medium supplemented with various concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D, 0.5-5.0 mg l) in combination with Kinetin (Kn, 0.5 mg l) and 3% sucrose. Various stages were observed during the development of somatic embryos, including globular, heart, and torpedo-stages. Torpedo stage embryos were separated from the explants and subcultured on medium supplemented with various concentrations of different plant growth regulators for maturation. Maximum percentage (55%) of somatic embryo germination and plantlet formation was found at 1.0 mg l BA. Finally, about 68% of plantlets were successfully established under field conditions. The regenerated plants were morphologically normal, fertile and able to set viable seeds.
在添加了不同浓度2,4-二氯苯氧乙酸（2,4-D，0.5 - 5.0毫克/升）、激动素（Kn，0.5毫克/升）和3%蔗糖的MS培养基上培养时，通过体细胞胚胎发生实现了[植物名称]子叶和叶片外植体的植株再生方案。在体细胞胚胎发育过程中观察到了各个阶段，包括球形、心形和鱼雷形阶段。将鱼雷形阶段的胚胎从外植体上分离下来，接种到添加了不同浓度不同植物生长调节剂的培养基上进行成熟培养。在1.0毫克/升苄氨基腺嘌呤（BA）时，体细胞胚胎萌发和植株形成的百分比最高（55%）。最后，约68%的植株在田间条件下成功移栽成活。再生植株形态正常、可育且能够结出有活力的种子。
