Tian Ju, Zhu Jialei, Deng Xiaohan, Zhu Xu, Wang Ruigang, Li Guojing
Inner Mongolia Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions, Inner Mongolia Agricultural University, Hohhot 010018, China.
School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, China.
Plants (Basel). 2025 May 21;14(10):1545. doi: 10.3390/plants14101545.
is a perennial shrub species in the genus (Fabaceae), demonstrating remarkable stress resistance and adaptability. However, research on its somatic embryogenesis (SE) and genetic transformation techniques remains limited. In this study, we established an SE system by utilizing immature cotyledons isolated from young seeds. Our findings demonstrated that the immature cotyledons at 6-7 weeks after flowering (WAF) were the best explants for SE. The optimal embryo induction medium consisted of an MS basal medium supplemented with 5 mg/L α-naphthaleneacetic acid (NAA), 3 mg/L 6-benzylaminopurine (6-BA), 30 g/L sucrose, 7 g/L agar, and 500 mg/L hydrolyzed casein. Cotyledon-stage embryos germinated on a half-strength MS medium, exhibiting a 34.36% germination rate. Based on the SE system, we developed a preliminary genetic transformation system using the reporter gene, which successfully generated transgenic calli and cotyledon-stage embryos. The establishment of the SE system is expected to shorten breeding cycles, facilitate propagation of superior cultivars, and support large-scale industrial applications in . Furthermore, the stable transformation system provides a platform for molecular breeding and gene function verification.
是豆科（Fabaceae）属的一种多年生灌木物种，具有显著的抗逆性和适应性。然而，对其体细胞胚胎发生（SE）和遗传转化技术的研究仍然有限。在本研究中，我们利用从幼嫩种子中分离的未成熟子叶建立了一个SE系统。我们的研究结果表明，开花后6 - 7周（WAF）的未成熟子叶是SE的最佳外植体。最佳胚胎诱导培养基由添加了5 mg/Lα-萘乙酸（NAA）、3 mg/L 6-苄基腺嘌呤（6-BA）、30 g/L蔗糖、7 g/L琼脂和500 mg/L水解酪蛋白的MS基本培养基组成。子叶期胚胎在1/2强度的MS培养基上萌发，萌发率为34.36%。基于SE系统，我们利用报告基因开发了一个初步的遗传转化系统，成功产生了转基因愈伤组织和子叶期胚胎。SE系统的建立有望缩短育种周期，促进优良品种的繁殖，并支持在……中的大规模工业应用。此外，稳定的转化系统为分子育种和基因功能验证提供了一个平台。
