Sari Eka Meutia, Jianlin Han, Noor Ronny Rachman, Sumantri Cece, Margawati Endang Tri
Dept. of Animal Science, Agriculture Faculty, Syiah Kuala University, Indonesia.
CAAS (Chinese Academy of Agricultural Sciences) - ILRI (International Livestock Research Institute) JLLFGR (Joint Laboratory on Livestock and Forage Genetic Resources), Beijing, China.
J Genet Eng Biotechnol. 2016 Jun;14(1):227-231. doi: 10.1016/j.jgeb.2015.12.001. Epub 2015 Dec 28.
The objective of this research was to find the basic data on genetic diversity of mtDNA D-Loop in Aceh cattle and its association with Bhutanese, Chinese, and Indian cattle. There were sixty samples of DNA which had been sequenced; i.e. Banda Aceh (11), Saree (20), and Indrapuri (29). To the best of our knowledge this is the first published data on the complete mitochondrial D-Loop sequence of Aceh cattle. Results show that Aceh cattle have the closest relationship to and have been influenced by . The closest genetic ranges among Aceh cattle, Bhutanese, Chinese, Indian and Zebu were Aceh-Zebu (0.0138), Aceh-Bhutanese (0.0156), Aceh-Chinese (0.0190) and Aceh-Indian (0.0193). D-Loop mtDNA analyses showed that there were 27 haplotypes in which twenty-one samples spread in haplotype 1, two samples were in haplotype 2, and the other four haplotypes had various samples in the range of three to seventeen samples. One sample of Aceh cattle from Saree has a closest maternal genetic with . One of the four mutations among the star-shaped clusters on median joining network was a new specific haploid-group in Aceh cattle. From this finding it could be assumed that Aceh cattle form a specific haplotype and it can be conclude that Aceh cattle are animal genetic resources from Aceh in Sumatera Island that have to be preserved.
本研究的目的是获取亚齐牛线粒体DNA D环区遗传多样性的基础数据，以及其与不丹牛、中国牛和印度牛的关联。共有60个DNA样本进行了测序，即班达亚齐（11个）、萨雷（20个）和因德拉普里（29个）。据我们所知，这是关于亚齐牛完整线粒体D环序列的首次公开数据。结果表明，亚齐牛与 关系最为密切，并受到了 的影响。亚齐牛、不丹牛、中国牛、印度牛和瘤牛之间最近的遗传距离分别为亚齐牛 - 瘤牛（0.0138）、亚齐牛 - 不丹牛（0.0156）、亚齐牛 - 中国牛（0.0190）和亚齐牛 - 印度牛（0.0193）。线粒体D环区分析显示，共有27种单倍型，其中21个样本分布在单倍型1中，2个样本在单倍型2中，其他4种单倍型的样本数量在3至17个之间。来自萨雷的一头亚齐牛样本与 具有最接近的母系遗传关系。在中介连接网络上的星形聚类中的四个突变之一是亚齐牛中的一个新的特定单倍体群。从这一发现可以推测，亚齐牛形成了一个特定的单倍型，并且可以得出结论，亚齐牛是苏门答腊岛亚齐的动物遗传资源，必须加以保护。 （注：原文中部分表述不完整，如“have the closest relationship to and have been influenced by.”以及“One sample of Aceh cattle from Saree has a closest maternal genetic with.”中缺少具体对象，翻译时保留原文形式。）
