Mouafi Foukia E, Ibrahim Ghada S, Abo Elsoud Mostafa M
Microbial Biotechnology Dept., Genetic Engineering and Biotechnology Division, National Research Centre, El-Bohouth Street, Dokki, Cairo, Egypt.
King Abdulaziz university, Saudi Arabia.
J Genet Eng Biotechnol. 2016 Dec;14(2):253-259. doi: 10.1016/j.jgeb.2016.10.006. Epub 2016 Nov 4.
The present investigation, focused on screening of various fungal species for Lovastatin production using different agro-based wastes, also, for maximizing lovastatin productivity by isolated using response surface methodology (RSM). The following substrates (Olive cake; Pea pods; sugarcane bagasse; wheat bran; rice hulls; beet peel; Potato peel and groundnut shells) were screened to evaluate their effectiveness for lovastatin production, using different fungal species, ( and isolated ) under solid state fermentation (SSF). Wheat bran was the most suitable substrate for lovastatin production with all fungal species. Optimum conditions of lovastatin production by wheat bran have been attained efficiently by response surface methodology (RSM) using isolated under solid state fermentation (SSF). The lovastatin yield of (3.353 mg/g DFM) was obtained at an optimum temperature of 28 °C; pH of 5.00; initial moisture content of 70% and incubation period of 12 days. This Lovastatin has the possibility to use in different therapeutic applications.
本研究聚焦于使用不同的农业废弃物筛选用于生产洛伐他汀的各种真菌物种，同时通过响应面法（RSM）分离以最大化洛伐他汀的产量。筛选了以下底物（橄榄饼；豌豆荚；甘蔗渣；麦麸；稻壳；甜菜皮；土豆皮和花生壳），以评估它们在固态发酵（SSF）条件下使用不同真菌物种生产洛伐他汀的有效性。麦麸是所有真菌物种生产洛伐他汀最合适的底物。通过响应面法（RSM）在固态发酵（SSF）条件下分离，已有效地获得了麦麸生产洛伐他汀的最佳条件。在最佳温度28°C、pH值5.00、初始水分含量70%和培养期12天的条件下，获得了洛伐他汀产量（3.353毫克/克干物质）。这种洛伐他汀有可能用于不同的治疗应用。
