Marzan Lolo Wal, Barua Rinty, Akter Yasmin, Arifuzzaman Md, Islam Md Rafiqul, Shimizu Kazuyuki
Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong - 4331, Bangladesh.
Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong, Bangladesh.
J Genet Eng Biotechnol. 2017 Jun;15(1):161-168. doi: 10.1016/j.jgeb.2017.01.004. Epub 2017 Feb 8.
Abundant, low prices and a highly reduced nature make glycerol to be an ideal feedstock for the production of reduced biochemicals and biofuels. has been paid much attention as the platform of microbial cell factories due to its high growth rate (giving higher metabolite production rate) and the capability of utilizing a wide range of carbon sources. However, one of the drawbacks of using as a platform is its mixed metabolite formation under anaerobic conditions. In the present study, it was shown that ethanol could be exclusively produced from glycerol by the wild type , while d-lactic acid could be exclusively produced from glucose by mutant, where the glucose uptake rate could be increased by this mutant as compared to the wild type strain. It was also shown that the growth rate is significantly reduced in mutant for the case of using glycerol as a carbon source due to redox imbalance. The metabolic regulation mechanisms behind the fermentation characteristic were clarified to some extent.
甘油产量丰富、价格低廉且性质高度还原，使其成为生产还原型生物化学品和生物燃料的理想原料。由于其高生长速率（可实现更高的代谢物生产率）以及利用多种碳源的能力，[具体微生物名称]作为微生物细胞工厂的平台受到了广泛关注。然而，将[具体微生物名称]用作平台的一个缺点是其在厌氧条件下会形成混合代谢物。在本研究中，结果表明野生型[具体微生物名称]可以仅从甘油中产生乙醇，而[具体微生物名称]突变体可以仅从葡萄糖中产生d - 乳酸，与野生型菌株相比，该突变体能够提高葡萄糖摄取速率。研究还表明，由于氧化还原失衡，在以甘油作为碳源的情况下，[具体微生物名称]突变体的生长速率显著降低。在一定程度上阐明了发酵特性背后的代谢调控机制。
