Kaithwas Vipin, Bhardwaj Krati, Gupta Durgesh, Bhargava Santosh
Division of Microbiology, Department of Botany, Government Motilal Science College, Bhopal, MP 462008, India.
Bioinformatics Centre, Barkatullah University, Bhopal, MP 462026, India.
J Genet Eng Biotechnol. 2017 Jun;15(1):231-237. doi: 10.1016/j.jgeb.2016.12.007. Epub 2017 Feb 2.
In this study the proteome response of the two diazotrophic organism's . and exposed to salt (NaCl) and osmotic (sucrose) stresses was compared. Out of the total over expressed proteins; we have selected only three over expressed proteins . GroEL chaperonin, nitrogenase Mo-Fe protein and argininosuccinate synthase for further analysis, and then we analyzed the amino acid frequencies of all the three over expressed proteins. That led to the conclusion that amino acids e.g. alanine, glycine and valine that were energetically cheaper to produce were showing higher frequencies. This study would help in tracing the phylogenetic relationship between protein families.
在本研究中，比较了两种固氮生物在暴露于盐（氯化钠）和渗透（蔗糖）胁迫下的蛋白质组反应。在所有上调表达的蛋白质中，我们仅选择了三种上调表达的蛋白质——GroEL伴侣蛋白、固氮酶钼铁蛋白和精氨酸琥珀酸合酶进行进一步分析，然后我们分析了这三种上调表达蛋白质的氨基酸频率。由此得出结论，例如丙氨酸、甘氨酸和缬氨酸等生产成本较低的氨基酸显示出较高的频率。这项研究将有助于追踪蛋白质家族之间的系统发育关系。
