Abdelrauf Lobna M, Abdel Rahman Mohamed F, Abdel-Maksoud Sahar M, Farag Nabil M, Hashad Ingy M
Clinical Biochemistry Unit, Faculty of Pharmacy and Biotechnology, The German University in Cairo, Egypt.
Department of Biochemistry, Faculty of Pharmacy, October University for Modern Sciences and Arts, Giza, Egypt.
J Genet Eng Biotechnol. 2017 Dec;15(2):415-418. doi: 10.1016/j.jgeb.2017.07.009. Epub 2017 Oct 3.
Oxidative stress has been implicated in various diseases including atherosclerosis; the most common pathologic process underlying acute myocardial infarction (AMI). The manganese superoxide dismutase (MnSOD) antioxidant enzyme affords the major defense against reactive oxygen species (ROS) within the mitochondria. MnSOD Alanine16Valine (A16V) single nucleotide polymorphism (SNP) has been shown to decrease MnSOD detoxification activity. A case-control study was conducted to investigate the association between MnSOD A16V polymorphism and the incidence of AMI in the Egyptians, investigate the contribution of oxidative stress represented by hexanoyl lysine adduct (HEL), an oxidative stress biomarker, in the pathogenesis of AMI and finally correlate the MnSOD genotypes with HEL serum levels. A total of 200 Egyptian subjects were recruited for the study; 100 AMI patients and 100 control subjects. Genotypes of the MnSOD A16V polymorphism were determined using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Serum HEL was measured by ELISA. A significant difference in the distribution of the MnSOD A16V genotypes was observed; VV genotype was significantly higher in AMI than controls ( ≤ 0.0001). Also, studying the allele frequencies revealed that Val allele was significantly higher in AMI than controls ( ≤ 0.0001). Serum analysis showed higher levels of HEL in AMI patients ( = 0.0142 Furthermore, HEL levels were found to be significantly higher in VV genotype in AMI ( = 0.0273). Our study suggests that MnSOD A16V polymorphism is associated with increased risk of developing AMI in the Egyptians. Moreover, the VV genotype is associated with higher HEL levels.
氧化应激与包括动脉粥样硬化在内的多种疾病有关；动脉粥样硬化是急性心肌梗死（AMI）最常见的病理过程。锰超氧化物歧化酶（MnSOD）抗氧化酶是线粒体中抵御活性氧（ROS）的主要防御机制。已证明MnSOD丙氨酸16缬氨酸（A16V）单核苷酸多态性（SNP）会降低MnSOD的解毒活性。本病例对照研究旨在调查埃及人群中MnSOD A16V多态性与AMI发病率之间的关联，研究氧化应激生物标志物己酰赖氨酸加合物（HEL）所代表的氧化应激在AMI发病机制中的作用，并最终将MnSOD基因型与HEL血清水平相关联。本研究共招募了200名埃及受试者；100名AMI患者和100名对照受试者。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）确定MnSOD A16V多态性的基因型。通过酶联免疫吸附测定（ELISA）测量血清HEL。观察到MnSOD A16V基因型的分布存在显著差异；AMI患者中VV基因型显著高于对照组（P≤0.0001）。此外，对等位基因频率的研究表明，AMI患者中缬氨酸等位基因显著高于对照组（P≤0.0001）。血清分析显示AMI患者的HEL水平较高（P = 0.0142）。此外，发现AMI患者中VV基因型的HEL水平显著更高（P = 0.0273）。我们的研究表明，MnSOD A16V多态性与埃及人发生AMI的风险增加有关。此外，VV基因型与更高的HEL水平相关。
