Mahmudunnabi Golam, Majlish Al Nahian Khan, Momtaz Farhana, Foysal Md Javed, Rahman Md Mahbubur, Islam Kamrul
Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet 3114, Bangladesh.
Department of Microbiology, University of Chittagong, Chittagong 4331, Bangladesh.
J Genet Eng Biotechnol. 2018 Jun;16(1):77-82. doi: 10.1016/j.jgeb.2017.12.004. Epub 2018 Jan 4.
is the second leading causative agent of UTI. In this study, a rapid combined polymerase chain reaction and restriction fragment length polymorphism analysis was developed to identify in women, infected with urinary tract infection in the Sylhet city of Bangladesh. Analysis of 11 isolates from women at the age range of 20-55 from three different hospitals were done firstly by amplification with specific ITS primers. All of the 11 collected isolates were amplified in PCR and showed the expected 136 bp products. Then, restriction fragment length polymorphism analysis of 11 isolates were conducted after PCR amplification by 16s rRNA universal primers, followed by subsequent digestion and incubation with two restriction enzymes, and . Seven out of 11 isolates were digested by restriction enzymes, six isolates digested by , and while others were negative for both enzymes. Data results reveal that, women at age between 25 and 50 were digested by both enzymes. A woman aged over than 50 was negative while bellow 20 was digested by only . The results could pave the tactic for further research in the detection of from UTI infected women.
是尿路感染的第二大致病因子。在本研究中，开发了一种快速聚合酶链反应和限制性片段长度多态性联合分析方法，用于鉴定孟加拉国锡尔赫特市患有尿路感染的女性中的[具体病原体名称未给出]。首先，使用特定的ITS引物对来自三家不同医院的年龄在20 - 55岁的11名女性分离株进行扩增分析。收集的11株分离株在PCR中均得到扩增，并显示出预期的136 bp产物。然后，用16s rRNA通用引物进行PCR扩增后，对11株分离株进行限制性片段长度多态性分析，随后用两种限制性内切酶[酶名称未给出]和[酶名称未给出]进行消化和孵育。11株分离株中有7株被[酶名称未给出]限制性内切酶消化，6株被[酶名称未给出]消化，而其他分离株对两种酶均呈阴性。数据结果显示，年龄在25至50岁之间的女性被两种酶消化。一名50岁以上的女性呈阴性，而20岁以下的女性仅被[酶名称未给出]消化。这些结果可为进一步研究检测尿路感染女性中的[具体病原体名称未给出]奠定策略基础。
