Yang Kebin, Li Ying, Wang Sining, Xu Xiurong, Sun Huayu, Zhao Hansheng, Li Xueping, Gao Zhimin
Institute of Gene Science for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing, China.
State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Beijing, China.
PeerJ. 2019 Jan 11;6:e6242. doi: 10.7717/peerj.6242. eCollection 2019.
The MYB family, one of the largest transcription factor (TF) families in the plant kingdom, plays vital roles in cell formation, morphogenesis and signal transduction, as well as responses to biotic and abiotic stresses. However, the underlying function of bamboo MYB TFs remains unclear. To gain insight into the status of these proteins, a total of 85 PeMYBs, which were further divided into 11 subgroups, were identified in moso bamboo () by using a genome-wide search strategy. Gene structure analysis showed that s were significantly different, with exon numbers varying from 4 to 13. Phylogenetic analysis indicated that PeMYBs clustered into 27 clades, of which the function of 18 clades has been predicted. In addition, almost all of the s were differently expressed in leaves, panicles, rhizomes and shoots based on RNA-seq data. Furthermore, qRT-PCR analysis showed that 12 s related to the biosynthesis and deposition of the secondary cell wall (SCW) were constitutively expressed, and their transcript abundance levels have changed significantly with increasing height of the bamboo shoots, for which the degree of lignification continuously increased. This result indicated that these s might play fundamental roles in SCW thickening and bamboo shoot lignification. The present comprehensive and systematic study on the members of the MYB family provided a reference and solid foundation for further functional analysis of MYB TFs in moso bamboo.
MYB家族是植物界最大的转录因子（TF）家族之一，在细胞形成、形态发生和信号转导以及对生物和非生物胁迫的响应中发挥着至关重要的作用。然而，竹子MYB转录因子的潜在功能仍不清楚。为了深入了解这些蛋白质的情况，利用全基因组搜索策略在毛竹中鉴定出了总共85个PeMYB，它们进一步被分为11个亚组。基因结构分析表明，这些基因存在显著差异，外显子数量从4到13不等。系统发育分析表明，PeMYB聚为27个分支，其中18个分支的功能已被预测。此外，根据RNA测序数据，几乎所有这些基因在叶片、圆锥花序、根茎和笋中均有不同程度的表达。此外，qRT-PCR分析表明，12个与次生细胞壁（SCW）生物合成和沉积相关的基因组成型表达，并且随着竹笋高度的增加，它们的转录丰度水平发生了显著变化，竹笋的木质化程度也持续增加。这一结果表明，这些基因可能在SCW增厚和竹笋木质化过程中发挥重要作用。本研究对MYB家族成员进行了全面系统的研究，为进一步对毛竹MYB转录因子进行功能分析提供了参考和坚实基础。
