Wong H, Chang T M
Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, PQ, Canada.
Biomater Artif Cells Artif Organs. 1988;16(4):731-9. doi: 10.3109/10731198809117565.
Viable hepatocytes were microencapsulated within artificial cells to form a bio-artificial liver. Trypan blue stain exclusion testing generally showed that 60% of the encapsulated hepatocytes remained viable immediately after encapsulation. To determine if xenogeneic hepatocytes can be successfully transplanted, encapsulated rat hepatocytes were implanted intra-peritoneally into mice and galactosamine induced liver failure mice. After 29 days of implantation in mice and 8 days of implantation in liver failure induced mice there was no significant change observed in the number of hepatocytes within the free floating microcapsules recovered from the peritoneal cavity. The percentage viability of the hepatocytes inside the recovered microcapsules increased from 62% to nearly 100% after 29 days of implantation in mice. The percentage viability of the encapsulated hepatocytes implanted in the liver failure induced mice was followed for 8 days. The percentage viability of the hepatocytes inside the microcapsule also increased from 42% to nearly 100%. In mice implanted with free rat hepatocytes, no viable hepatocytes were observed as early as 4 and 5 days after implantation. Instead a larger number of lymphoid cells and remnants of hepatocytes were recovered from the peritoneal cavity. In all cases the viability of the rat hepatocytes were determined with trypan blue stain.
将有活力的肝细胞微囊化于人工细胞内以形成生物人工肝。台盼蓝染色排除试验通常显示，在微囊化后立即有60%的被包封肝细胞保持存活。为了确定异种肝细胞能否成功移植，将包封的大鼠肝细胞腹腔内植入小鼠和半乳糖胺诱导的肝衰竭小鼠体内。在小鼠体内植入29天以及在肝衰竭诱导小鼠体内植入8天后，从腹腔回收的游离漂浮微囊内的肝细胞数量未观察到显著变化。在小鼠体内植入29天后，回收微囊内肝细胞的存活百分比从62%增加至近100%。对植入肝衰竭诱导小鼠体内的包封肝细胞的存活百分比进行了8天的跟踪。微囊内肝细胞的存活百分比也从42%增加至近100%。在植入游离大鼠肝细胞的小鼠中，早在植入后4天和5天就未观察到有活力的肝细胞。相反，从腹腔回收了大量淋巴细胞和肝细胞残余物。在所有情况下，大鼠肝细胞的活力均通过台盼蓝染色来确定。
