用于生物人工肝支持的微囊化肝细胞
Microencapsulated hepatocytes for bioartificial liver support.
作者信息
Cai Z H, Shi Z Q, O'Shea G M, Sun A M
机构信息
University of Toronto, Department of Physiology, Ontario, Canada.
出版信息
Artif Organs. 1988 Oct;12(5):388-93. doi: 10.1111/j.1525-1594.1988.tb02793.x.
Free hepatocytes, harvested from normal rat livers by portal vein collagenase perfusion, were encapsulated within alginate-polylysine membranes and served as a liver support system. The encapsulated hepatocytes remained viable and were able to synthesize protein for up to 3 weeks in culture. Allografts of encapsulated hepatocytes replaced the function of a damaged liver and reduced the mortality rate among rats with galactosamine-induced fulminant hepatic failure.
通过门静脉胶原酶灌注从正常大鼠肝脏中获取的游离肝细胞，被包裹在藻酸盐 - 聚赖氨酸膜内，用作肝脏支持系统。被包裹的肝细胞保持存活，并能够在培养中合成蛋白质长达3周。被包裹肝细胞的同种异体移植替代了受损肝脏的功能，并降低了半乳糖胺诱导的暴发性肝衰竭大鼠的死亡率。
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