Peptide Institute, Inc., Ibaraki, Osaka 567-0085, Japan.
Org Biomol Chem. 2019 Jan 31;17(5):1202-1205. doi: 10.1039/c8ob03098g.
Solubilizing trityl tags (Trt-oligoLys/Arg) proved applicable to metal-free radical-triggered desulfurization and an Ag-mediated thioester method. Additionally, using the solubilizing trityl tag strategy, synthesis of the influenza BM2 proton channel, which previously required organic solvent-aided native chemical ligation (NCL) and desulfurization due to its low solubility, was achieved without using organic solvents.
证明了三苯甲基化标签（Trt-寡聚赖氨酸/精氨酸）在无金属自由基引发的脱硫和 Ag 介导的硫酯方法中是适用的。此外，通过使用可溶性三苯甲基化标签策略，成功合成了流感 BM2 质子通道，由于其低溶解度，以前需要有机溶剂辅助的天然化学连接（NCL）和脱硫才能实现，现在无需使用有机溶剂。
