Peptide Institute, Inc., 7-2-9 Saito-Asagi, Ibaraki-Shi, Osaka, 567-0085, Japan.
Chembiochem. 2019 Aug 1;20(15):1906-1913. doi: 10.1002/cbic.201900105. Epub 2019 May 27.
Recently, solubilizing tag methods (Trt-K and Trt-R method) were developed for the challenging synthesis of peptides/proteins by means of native chemical ligation. In this system, the solubilizing tag can be attached to the Cys side chain by simply mixing the tag-introducing reagent under acidic conditions. The tagged peptides/proteins exhibited high water solubility thanks to the introduction of redundant oligo-Lys/Arg. In the final reaction, the tag can be quickly and cleanly detached by a standard deprotection reaction with trifluoroacetic acid. Herein, the development and application of these methods are described.
最近，为了通过天然化学连接挑战性地合成肽/蛋白质，开发了增溶标签方法（Trt-K 和 Trt-R 方法）。在该系统中，通过在酸性条件下简单混合标签引入试剂，可以将增溶标签连接到 Cys 侧链上。由于引入了冗余的寡-Lys/Arg，标记的肽/蛋白质具有高水溶性。在最后的反应中，通过三氟乙酸的标准脱保护反应可以快速、干净地去除标签。本文描述了这些方法的发展和应用。
