小鼠和人类中调节性B细胞的鉴定与分离

Identification and Isolation of Regulatory B Cells in Mouse and Human.

作者信息

Menon Madhvi, Rosser Elizabeth C, Mauri Claudia

机构信息

Centre for Rheumatology, Division of Medicine, University College London, 5 University Street, London, WC1E 6JF, UK.

出版信息

Methods Mol Biol. 2019;1899:55-66. doi: 10.1007/978-1-4939-8938-6_5.

DOI:10.1007/978-1-4939-8938-6_5

PMID:30649765

Abstract

Regulatory B cells (Bregs) suppress immune response via the provision of IL-10. Due to the phenotypic heterogeneity of described Bregs, it is important to have standardized protocols for their isolation and identification. Previous work by our laboratory has shown that the immature B-cell populations in the murine spleen and human peripheral blood produce the highest levels of IL-10 on engagement of CD40, and can suppress pro-inflammatory T-cell differentiation. In this chapter, we describe the methods necessary for the isolation of this subset of Bregs and their activation via CD40 in vitro.

摘要

调节性B细胞（Bregs）通过分泌白细胞介素-10（IL-10）来抑制免疫反应。由于所描述的Bregs存在表型异质性，因此制定标准化的分离和鉴定方案非常重要。我们实验室之前的工作表明，小鼠脾脏和人类外周血中的未成熟B细胞群体在CD40激活时产生的IL-10水平最高，并且能够抑制促炎性T细胞分化。在本章中，我们描述了分离该Bregs亚群并在体外通过CD40激活它们所需的方法。