Cao Z H, Huang S, Chen R, Wu W Y
Capital University of Medical Sciences, Beijing.
Zhonghua Zhong Liu Za Zhi. 1988 May;10(3):188-90.
Sections of human placental villi tissue and carcinoma cell lines (SMMC-7721, (MGC-803 and Kato-111; were examined using the indirect immunoperoxidase histological technique to further investigate the localization of the tumor-associated placental protein (PP) in human placental tissue and the expression of PP on carcinoma cell lines. PP was prepared from placenta in the first trimester. Placental tissue sections exposed to PP antisera showed a marked staining of the trophoblast cells, especially in syncytiotrophoblast. The strongly stained area was localized to the cell membrane of syncytiotrophoblast. In contrast, the nucleus and the cytoplasm of syncytiotrophoblast were stained weakly. The specific PP antisera also stained the carcinoma cell lines. Among different carcinoma cell lines, the degree of staining of liver carcinoma cell line (SMMC-7721) was higher than that of gastric carcinoma cell lines (MGC-803 and Kato-111). In the carcinoma cell lines the presence of antigen similar to PP suggests that oncogene (one) occur in human trophoblast cells as well as mice trophoblast cells.
使用间接免疫过氧化物酶组织学技术检查了人胎盘绒毛组织切片和癌细胞系（SMMC-7721、MGC-803和Kato-III），以进一步研究肿瘤相关胎盘蛋白（PP）在人胎盘组织中的定位以及PP在癌细胞系上的表达。PP取自孕早期的胎盘。暴露于PP抗血清的胎盘组织切片显示滋养层细胞有明显染色，尤其是合体滋养层。强染色区域定位于合体滋养层的细胞膜。相比之下，合体滋养层的细胞核和细胞质染色较弱。特异性PP抗血清也对癌细胞系进行了染色。在不同的癌细胞系中，肝癌细胞系（SMMC-7721）的染色程度高于胃癌细胞系（MGC-803和Kato-III）。在癌细胞系中存在与PP相似的抗原表明癌基因在人滋养层细胞以及小鼠滋养层细胞中均有发生。
