Team Gliotransmission & Synaptopathies, Centre de Recherche en Neurobiologie et Neurophysiologie de Marseille UMR7286 CNRS, Aix Marseille University, Marseille, France.
COMETE U1075 INSERM-Unicaen, University of Caen Normandie, Caen, France.
Acta Physiol (Oxf). 2019 May;226(1):e13257. doi: 10.1111/apha.13257. Epub 2019 Feb 14.
The last two decades have witnessed remarkable advance in our understanding the role of d-amino acids in the mammalian nervous system: from the unknown, to known molecules with unknown functions, to potential central players in health and disease. d-Amino acids have emerged as an important class of signaling molecules. In particular, the exploration of the roles of d-serine in brain physiopathology is a vibrant field that is growing at an accelerating pace. However, disentangling the functions of a chiral molecule in a complex chemical matrice as the brain requires specific measurement and detection methods but is also a challenging task as many molecular tools and models investigators are using can lead to confounded observations. Thus, study of d-amino acids demands accurate methodologies and specific controls, and these have often been lacking. Here we outline best practices for d-amino acid research, with a special emphasis on d-serine. We hope these concepts help move the field to greater rigor and reproducibility, allowing the field to advance.
在过去的二十年中，我们对 D-氨基酸在哺乳动物神经系统中的作用的理解取得了显著的进展：从未知到已知但功能未知的分子，再到健康和疾病中潜在的重要参与者。D-氨基酸已成为一类重要的信号分子。特别是，D-丝氨酸在大脑生理病理学中的作用的探索是一个充满活力的领域，其发展速度正在加快。然而，要在大脑这样的复杂化学基质中解明手性分子的功能，需要特定的测量和检测方法，但这也是一项具有挑战性的任务，因为研究人员使用的许多分子工具和模型可能会导致观察结果混乱。因此，D-氨基酸的研究需要准确的方法和具体的对照，而这些往往是缺乏的。在这里，我们概述了 D-氨基酸研究的最佳实践，特别强调了 D-丝氨酸。我们希望这些概念有助于提高该领域的严谨性和可重复性，从而推动该领域的发展。
