Xu Pai-di, Zhang Hong-Xing, Yang Yun, Xin Yu
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2017 Mar;37(3):360-364.
Objective To observe the effects of electroacupuncture (EA) on vasoactive intesti- nal peptide (VIP) , calcitonin gene-related peptide (CGRP) expression, gastric emptying, and small in- testine advance rate in functional dyspepsia (FD) rats. Methods Totally 48 SD rats were randomly di- vided into three groups, the blank group, the model group, and the EA group, 16 in each group. Except rats in the blank group, FD model was established by tail clamped stimulation plus irregular diet, and ice physiological saline gastrogavage for 14 successive days. After successful modeling EA at Zusanli (ST36) and Taichong (LR3) were performed, once per day for 28 days. Rats were intervened by gastro- gavage at the end of the treatment. Gastric tissue and small intestinal tissue were sampled after anatomy. The rates of gastric emptying and small intestinal transit were determined. Pathological changes of gastric antrum and jejunum tissue were observed by HE staining. mRNA expression levels of VIP and CGRP in gastric antrum and jejunum tissue were determined by Real-time PCR. Results No organic change oc- curred in tissues of the 3 groups. No gastric or intestinal ulcers , inflammatory infiltration, or glandular ep- ithelial lesion occurred in the 3 groups. Compared with the blank group, gastric residual rate obviously in- creased, small intestinal transit rate was lowered, mRNA expression levels of VIP and CGRP in gastric antrum and jejunum tissue were obviously elevated in the model group (P <0. 01, P <0. 05). Compared with the model group, gastric residual rate was obviously reduced, small intestinal transit was obviously elevated, mRNA expression levels of VIP and CGRP in gastric antrum and jejunum tissue were obviously decreased (P <0. 05, P <0. 01). Conclusions EA could significantly decrease mRNA expressions of VIP and CGRP in gastrointestinal tract, accelerate gastric emptying rate and small intestinal transit rate. EA's improving the gastrointestinal motility might be related to decreasing mRNA expressions of VIP and CGRP in gastrointestinal tract, indicating that abnormal secretion braingut peptide might be one of important mechanisms for FD.
目的 观察电针（EA）对功能性消化不良（FD）大鼠血管活性肠肽（VIP）、降钙素基因相关肽（CGRP）表达、胃排空及小肠推进率的影响。方法 将48只SD大鼠随机分为空白组、模型组和电针组，每组16只。除空白组大鼠外，采用夹尾刺激加不规则饮食的方法建立FD模型，并连续14天给予冰生理盐水灌胃。造模成功后，针刺足三里（ST36）和太冲（LR3），每天1次，共28天。治疗结束时通过灌胃对大鼠进行干预。解剖后取胃组织和小肠组织。测定胃排空率和小肠传输率。通过苏木精-伊红（HE）染色观察胃窦和空肠组织的病理变化。采用实时荧光定量聚合酶链反应（Real-time PCR）测定胃窦和空肠组织中VIP和CGRP的mRNA表达水平。结果 3组大鼠组织均未出现器质性改变。3组大鼠均未出现胃溃疡、炎症浸润或腺上皮病变。与空白组相比，模型组胃残留率明显升高，小肠传输率降低，胃窦和空肠组织中VIP和CGRP的mRNA表达水平明显升高（P<0.01，P<0.05）。与模型组相比，电针组胃残留率明显降低，小肠传输率明显升高，胃窦和空肠组织中VIP和CGRP的mRNA表达水平明显降低（P<0.05，P<0.01）。结论 电针可显著降低胃肠道中VIP和CGRP的mRNA表达，加快胃排空率和小肠传输率。电针改善胃肠动力可能与降低胃肠道中VIP和CGRP的mRNA表达有关，提示脑肠肽分泌异常可能是FD的重要发病机制之一。
