Wang Xiao-Yan, Li Chun-Yu, Zhang Ya-Nan, Fu Chen-Yu, Gao Shan-Shan, Wang Shi-Jun
College of Acupuncture-moxibustion and Massage, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
Department of Traditional Chinese Medicine, Qingdao West Coast New Area District Hospital, Qingdao 266000, Shandong Province.
Zhen Ci Yan Jiu. 2021 Jul 25;46(7):549-54. doi: 10.13702/j.1000-0607.200107.
To observe the effect of electroacupuncture(EA) at "Zusanli"(ST36), "Yinlingquan" (SP9) or "Yingu"(KI10) on the expression of 5-hydroxytryptamine type 7 receptor (5-HT7R) in the gastric antrum and colon tissues in functional diarrhea (FD) model rats, so as to explore its mechanisms underlying improving FD.
Forty male SD rats were randomly divided into control, model, ST36, SP9 and KI10 groups，with 8 rats in each group. The FD model was established by combined administration of restriction (four-limbs' banding) + abdominal cold stimulation + feeding every other day, for 14 days. EA (2 Hz, 0.5 mA) was applied to bilateral ST36 or bilateral SP9 or bilateral KI10 in the 3 corresponding groups for 30 min, once a day for 7 days after successful modeling. Rats of the control group received restriction only. The fecal water content was calculated and the stool form score was given according to the Bristol's methods. The gastric residual rate (GRR) and small intestine propulsion rate (SIPR) were determined to assess the motility of the gastrointestinal tract. Immunohistochemical and real-time fluorescent quantitative PCR were used to detect the expression of 5-HT7R protein and mRNA of the gastric antrum and colon tissues, respectively.
Compared with the control group, the fecal water content, the stool form score, the SIPR and the expression levels of 5-HT7R protein and 5-HT7R mRNA were significantly increased (<0.01,<0.05) and the GRR was considerably decreased in the model group (<0.01). The fecal water content, stool form score and SIPR, and expression level of 5-HT7R protein and mRNA in the gastric antrum and colon were significantly lower in both the ST36 and SP9 groups (not in the KI10 group) than in the model group (<0.01, <0.05), but the GRR was significantly higher in the ST36 and SP9 groups (not in the KI10 group) than in the model group (<0.01). The effects of both ST36 and SP9 were significantly superior to those of KI10 in improving all the indexes mentioned above （except SIPR and the mRNA level of 5-HT7R in the colon in SP9 group）(<0.01, <0.05). No significant differences were found between the ST36 and SP9 groups in lowering the levels of fecal water content, stool form score, SIPR, and the expression of 5-HT7R protein and mRNA, as well as in up-regulating GRR (>0.05).
EA of ST36 and SP9 can improve the motility of gastrointestinal tract in FD rats, which may be related to its functions in down-regulating the expression of 5-HT7R protein and mRNA in gastric antrum and colon tissues. The effects of ST36 and SP9 were obviously better than those of KI10 in ameliorating the gastric and intestinal motility (except GRR) and in lowering the expression of 5-HT7R protein and mRNA.
观察电针“足三里”（ST36）、“阴陵泉”（SP9）或“阴谷”（KI10）对功能性腹泻（FD）模型大鼠胃窦和结肠组织中5-羟色胺7型受体（5-HT7R）表达的影响，以探讨其改善FD的作用机制。
将40只雄性SD大鼠随机分为对照组、模型组、ST36组、SP9组和KI10组，每组8只。采用限制（四肢捆绑）+腹部冷刺激+隔日喂食的方法联合造模14天，建立FD模型。造模成功后，对相应的3组大鼠双侧ST36或双侧SP9或双侧KI10施加电针（2 Hz，0.5 mA）30分钟，每天1次，共7天。对照组大鼠仅进行限制处理。计算粪便含水量，并根据布里斯托方法给出粪便形态评分。测定胃残留率（GRR）和小肠推进率（SIPR），以评估胃肠道的运动功能。分别采用免疫组织化学和实时荧光定量PCR检测胃窦和结肠组织中5-HT7R蛋白和mRNA的表达。
与对照组相比，模型组粪便含水量、粪便形态评分、SIPR以及5-HT7R蛋白和5-HT7R mRNA表达水平显著升高（<0.01，<0.05），GRR显著降低（<0.01）。ST36组和SP9组（KI10组未出现）的粪便含水量、粪便形态评分和SIPR，以及胃窦和结肠中5-HT7R蛋白和mRNA表达水平均显著低于模型组（<0.01，<0.05），但ST36组和SP9组（KI10组未出现）的GRR显著高于模型组（<0.01）。在改善上述所有指标（SP9组结肠中SIPR和5-HT7R mRNA水平除外）方面，ST36组和SP9组的效果均显著优于KI10组（<0.01，<0.05）。在降低粪便含水量、粪便形态评分、SIPR以及5-HT7R蛋白和mRNA表达水平，以及上调GRR方面，ST36组和SP9组之间未发现显著差异（>0.05）。
ST36和SP9电针可改善FD大鼠的胃肠道运动功能，这可能与其下调胃窦和结肠组织中5-HT7R蛋白和mRNA表达有关。在改善胃肠动力（GRR除外）和降低5-HT7R蛋白和mRNA表达方面，ST36和SP9的效果明显优于KI10。
