Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan.
National Institute for Basic Biology, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan.
Development. 2019 Jan 16;146(2):dev159343. doi: 10.1242/dev.159343.
Cell morphology changes dynamically during embryogenesis, and these changes create new interactions with surrounding cells, some of which are presumably mediated by intercellular signaling. However, the effects of morphological changes on intercellular signaling remain to be fully elucidated. In this study, we examined the effect of morphological changes in Wnt-producing cells on intercellular signaling in the spinal cord. After mid-gestation, roof-plate cells stretched along the dorsoventral axis in the mouse spinal cord, resulting in new contact at their tips with the ependymal cells that surround the central canal. Wnt1 and Wnt3a were produced by the stretched roof-plate cells and delivered to the cell process tip. Whereas Wnt signaling was activated in developing ependymal cells, Wnt activation in dorsal ependymal cells, which were close to the stretched roof plate, was significantly suppressed in embryos with roof plate-specific conditional knockout of , which encodes a factor that is essential for Wnt secretion. Furthermore, proliferation of these cells was impaired in conditional knockout mice during development and after induced spinal cord injury in adults. Therefore, morphological changes in Wnt-producing cells appear to generate new Wnt signal targets.
胚胎发生过程中细胞形态发生动态变化，这些变化与周围细胞产生新的相互作用，其中一些可能是通过细胞间信号转导介导的。然而，形态变化对细胞间信号转导的影响仍有待充分阐明。在这项研究中，我们研究了 Wnt 产生细胞形态变化对脊髓中细胞间信号转导的影响。妊娠中期后，小鼠脊髓中的顶板细胞沿背腹轴伸展，导致其尖端与环绕中央管的室管膜细胞新接触。伸展的顶板细胞产生 Wnt1 和 Wnt3a，并传递到细胞过程的尖端。虽然 Wnt 信号在发育中的室管膜细胞中被激活，但在编码对 Wnt 分泌至关重要的因子的顶板特异性条件敲除的胚胎中，靠近伸展的顶板的背侧室管膜细胞中的 Wnt 激活被显著抑制。此外，在发育过程中和成年后诱导的脊髓损伤后，这些细胞的增殖在 条件敲除小鼠中受损。因此，Wnt 产生细胞的形态变化似乎产生了新的 Wnt 信号靶标。
