Mahan M J, Roth J R
Department of Biology, University of Utah, Salt Lake City 84112.
Genetics. 1988 Sep;120(1):23-35. doi: 10.1093/genetics/120.1.23.
We describe a genetic system for studying the reciprocality of chromosomal recombination; all substrates and recombination functions involved are provided exclusively by the bacterial chromosome. The genetic system allows the recovery of both recombinant products from a single recombination event. The system was used to demonstrate the full reciprocality of three different types of recombination events: (1) intrachromosomal recombination between direct repeats, causing deletions; (2) intrachromosomal recombination between inverse homologies, causing inversion of a segment of the bacterial chromosome; and (3) circle to circle recombination (in the absence of any plasmid or phage functions). Results suggest that intrachromosomal recombination in bacteria is frequently fully reciprocal.
我们描述了一种用于研究染色体重组相互性的遗传系统；所有涉及的底物和重组功能均仅由细菌染色体提供。该遗传系统允许从单个重组事件中回收两种重组产物。该系统用于证明三种不同类型重组事件的完全相互性：（1）直接重复序列之间的染色体内重组，导致缺失；（2）反向同源序列之间的染色体内重组，导致细菌染色体片段的倒位；以及（3）环到环重组（在没有任何质粒或噬菌体功能的情况下）。结果表明，细菌中的染色体内重组通常是完全相互的。
