Karnam Shyamala, Girish H C, Murgod Sanjay, Nayak Vaidhehi Narayan, Varsha V K, Yanduri Sarita
Department of Oral Pathology and Microbiology, Rajarajeswari Dental College and Hospital, Bengaluru, Karnataka, India.
Department of Oral Pathology and Microbiology, DAPMRV Dental College and Hospital, Bengaluru, Karnataka, India.
J Oral Maxillofac Pathol. 2018 Sep-Dec;22(3):443. doi: 10.4103/jomfp.JOMFP_228_17.
Tissue processing involves transition of the biopsy tissue in graded concentration of various chemicals to make the tissue amiable for sectioning. The entire process takes 2-3 working days before a microscopic slide is ready for diagnosis. In order to shorten the turnaround time, rapid tissue processing method using methyl salicylate was developed.
The aim of this study is to develop a rapid tissue processing technique using methyl salicylate as a clearing agent and to compare it with routine tissue processing technique.
A total of 70 tissue specimens were cut into two equal halves. One each was processed by routine processing technique (RoPT) and rapid processing technique (RaPT). Tissue specimens were measured before and after processing. Quality of staining and cellular-level shrinkage were observed and scored for specimens. Statistical analysis using Welch's unequal variances -test was performed. Costs of chemicals in both the techniques were compared to see the cost-effectiveness of RaPT.
Outcomes of both the processing techniques were comparable with statistically not significant values for all the parameters. Hence, the results of RaPT technique are satisfactory, and the use of this technique may prove beneficial to pathology laboratories.
组织处理涉及将活检组织置于不同浓度的各种化学试剂中进行过渡处理，以使组织便于切片。在制备好用于诊断的显微玻片之前，整个过程需要2至3个工作日。为了缩短周转时间，开发了使用水杨酸甲酯的快速组织处理方法。
本研究的目的是开发一种使用水杨酸甲酯作为透明剂的快速组织处理技术，并将其与常规组织处理技术进行比较。
总共将70个组织标本切成两半。每一半分别采用常规处理技术（RoPT）和快速处理技术（RaPT）进行处理。在处理前后对组织标本进行测量。观察标本的染色质量和细胞水平的收缩情况并进行评分。使用韦尔奇不等方差检验进行统计分析。比较两种技术中化学试剂的成本，以了解快速处理技术的成本效益。
两种处理技术的结果具有可比性，所有参数的统计学值均无显著差异。因此，快速处理技术的结果令人满意，该技术的应用可能对病理实验室有益。
