锌补充剂对 Wistar 大鼠亚慢性经口暴露于草甘膦基除草剂（GOBARA®）引起的免疫毒性的影响。
Effect of zinc supplementation on immunotoxicity induced by subchronic oral exposure to glyphosate-based herbicide (GOBARA®) in Wistar rats.
机构信息
Department of Veterinary Microbiology and Pathology, Faculty of Veterinary Medicine, University of Jos, Jos, Plateau State, Nigeria.
Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Borno State, Nigeria.
出版信息
J Int Med Res. 2023 Jan;51(1):3000605221147188. doi: 10.1177/03000605221147188.
OBJECTIVES
To evaluate the effect of zinc supplementation on immunotoxicity induced by subchronic oral exposure to glyphosate-based herbicide (GBH).
METHODS
Sixty adult male Wistar rats randomly divided equally into six groups were exposed to GBH by gavage daily for 16 weeks with or without zinc pretreatment. Group DW rats received distilled water (2 mL/kg), group Z rats received zinc (50 mg/kg), and group G1 and G2 rats received 187.5 and 375 mg/kg GBH, respectively. Group ZG1 and ZG2 rats were pretreated with 50 mg/kg zinc before exposure to 187.5 and 375 mg/kg GBH, respectively. Tumor necrosis factor alpha (TNF-α) and immunoglobulin (IgG, IgM, IgE) levels were measured by enzyme-linked immunosorbent assay. Spleen, submandibular lymph node, and thymus samples were processed for histopathology.
RESULTS
Exposure to GBH (G1 and G2) significantly increased serum TNF-α concentrations and significantly decreased serum IgG and IgM concentrations compared with the control levels. Moderate-to-severe lymphocyte depletion occurred in the spleen, lymph nodes, and thymus in the GBH-exposed groups. Zinc supplementation mitigated the immunotoxic effects of GBH exposure.
CONCLUSIONS
GBH exposure increased pro-inflammatory cytokine responses, decreased immunoglobulin production, and depleted lymphocytes in lymphoid organs in rats, but zinc supplementation mitigated this immunotoxicity.
目的
评估锌补充剂对慢性口服暴露于草甘膦基除草剂（GBH）引起的免疫毒性的影响。
方法
将 60 只成年雄性 Wistar 大鼠随机分为 6 组，每组 10 只，通过灌胃每天暴露于 GBH 16 周，同时或不进行锌预处理。DW 组大鼠给予蒸馏水（2mL/kg），Z 组大鼠给予锌（50mg/kg），G1 和 G2 组大鼠分别给予 187.5 和 375mg/kg 的 GBH。ZG1 和 ZG2 组大鼠在暴露于 187.5 和 375mg/kg 的 GBH 之前，分别用 50mg/kg 的锌进行预处理。通过酶联免疫吸附试验测量肿瘤坏死因子-α（TNF-α）和免疫球蛋白（IgG、IgM、IgE）水平。处理脾脏、颌下淋巴结和胸腺样本进行组织病理学检查。
结果
与对照组相比，暴露于 GBH（G1 和 G2）会显著增加血清 TNF-α 浓度，显著降低血清 IgG 和 IgM 浓度。在暴露于 GBH 的各组中，脾脏、淋巴结和胸腺中的淋巴细胞明显减少。锌补充剂减轻了 GBH 暴露引起的免疫毒性。
结论
GBH 暴露会增加促炎细胞因子的反应，降低免疫球蛋白的产生，并消耗大鼠淋巴器官中的淋巴细胞，但锌补充剂减轻了这种免疫毒性。
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