Rohr L R, Layfield L J, Wallin D, Hardy D
Department of Pathology, University of Utah, Salt Lake City, and Associated Regional and University Pathologists (ARUP), Salt Lake City, USA.
Am J Clin Pathol. 2001 May;115(5):703-8. doi: 10.1309/15fb-fld1-408x-jqa3.
Rapid processing of histopathologic material is becoming increasingly desirable to fulfill the needs of clinicians treating acutely ill patients. Traditional techniques for rapid processing of paraffin-embedded tissues require 4 to 5 hours, delaying treatment for some critically ill patients and requiring additional shifts of technologists in the laboratory. Microwave processing further shortens this time, allowing even more rapid histopathologic diagnosis. Few data exist comparing quality of microwave-processed tissue with that processed by more traditional techniques. We randomly selected 158 paired specimens from 111 patients. One member of the pair was processed routinely overnight, while the other was processed by the rapid microwave technique. The slides then were compared for quality of histologic preparation in a blinded fashion by 2 pathologists. Eight routinely processed specimens were judged as suboptimal, while 6 microwave-processed specimens were judged as suboptimal and 1 was considered unsatisfactory for evaluation. In the remaining cases, the material obtained by the 2 techniques was considered of identical quality. Microwave processing considerably shortens the preparation time for permanent histologic sections without a demonstrable decrease in section quality or "readability."
为满足治疗急重症患者的临床医生的需求，对组织病理学材料进行快速处理变得越来越必要。传统的石蜡包埋组织快速处理技术需要4至5小时，这会延误一些重症患者的治疗，并且需要实验室技术人员增加轮班。微波处理进一步缩短了这一时间，使得组织病理学诊断能够更快完成。很少有数据比较微波处理组织与传统技术处理组织的质量。我们从111名患者中随机选取了158对标本。每对标本中的一个采用常规过夜处理，另一个采用快速微波技术处理。然后由2名病理学家以盲法比较切片的组织学制备质量。8个常规处理的标本被判定为次优，而6个微波处理的标本被判定为次优，1个被认为无法用于评估。在其余病例中，两种技术获得的材料被认为质量相同。微波处理大大缩短了永久性组织切片的制备时间，且切片质量或“可读性”没有明显下降。
