Klein Hannah L, Ang Kenny K H, Arkin Michelle R, Beckwitt Emily C, Chang Yi-Hsuan, Fan Jun, Kwon Youngho, Morten Michael J, Mukherjee Sucheta, Pambos Oliver J, El Sayyed Hafez, Thrall Elizabeth S, Vieira-da-Rocha João P, Wang Quan, Wang Shuang, Yeh Hsin-Yi, Biteen Julie S, Chi Peter, Heyer Wolf-Dietrich, Kapanidis Achillefs N, Loparo Joseph J, Strick Terence R, Sung Patrick, Van Houten Bennett, Niu Hengyao, Rothenberg Eli
New York University School of Medicine, Department of Biochemistry and Molecular Pharmacology, New York, NY 10016, USA.
Small Molecule Discovery Center and Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143, USA.
Microb Cell. 2019 Jan 7;6(1):65-101. doi: 10.15698/mic2019.01.665.
Genomes are constantly in flux, undergoing changes due to recombination, repair and mutagenesis. , many of such changes are studies using reporters for specific types of changes, or through cytological studies that detect changes at the single-cell level. Single molecule assays, which are reviewed here, can detect transient intermediates and dynamics of events. Biochemical assays allow detailed investigation of the DNA and protein activities of each step in a repair, recombination or mutagenesis event. Each type of assay is a powerful tool but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.
基因组处于不断变化之中，由于重组、修复和诱变而发生改变。许多此类变化是通过针对特定类型变化的报告基因进行研究，或者通过在单细胞水平检测变化的细胞学研究来进行的。本文所综述的单分子检测方法能够检测瞬时中间体和事件动态。生化检测方法允许对修复、重组或诱变事件中每一步的DNA和蛋白质活性进行详细研究。每种检测方法都是一种强大的工具，但每种方法都有其独特的优点和局限性。本文对最常用的检测方法进行了综述、讨论，并作为未来研究的指南呈现。
