Sander Jeffry D
Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA.
Methods Mol Biol. 2019;1931:155-168. doi: 10.1007/978-1-4939-9039-9_11.
The application of CRISPR/Cas to introduce targeted genomic edits is powering research and discovery across the genetic frontier. Applying CRISPR/Cas in sorghum can facilitate the study of gene function and unlock our understanding of this robust crop that serves as a staple for some of the most food insecure regions on the planet. When paired with recent advances in sorghum tissue culture and Agrobacteria technology, CRISPR/Cas can be used to introduce desirable changes and natural genetic variations directly into agriculturally relevant sorghum lines facilitating product development. This chapter describes CRISPR/Cas gene editing and provides high-level strategies and expectations for applying this technology using Agrobacterium in sorghum.
CRISPR/Cas技术在基因组中引入靶向编辑的应用正在推动整个基因前沿领域的研究与发现。在高粱中应用CRISPR/Cas技术有助于基因功能的研究，并加深我们对这种重要作物的理解，高粱是地球上一些粮食最不安全地区的主食。当与高粱组织培养和农杆菌技术的最新进展相结合时，CRISPR/Cas可用于将理想的变化和自然遗传变异直接引入与农业相关的高粱品系中，从而促进产品开发。本章介绍了CRISPR/Cas基因编辑，并提供了使用农杆菌在高粱中应用该技术的高层次策略和预期。
