Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 4155 Genome Sciences Building, 250 Bell Tower Drive, Chapel Hill, NC, USA.
Plant Cell Physiol. 2018 Aug 1;59(8):1608-1620. doi: 10.1093/pcp/pcy079.
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system is a genome editing technology transforming the field of plant biology by virtue of the system's efficiency and specificity. The system has quickly evolved for many diverse applications including multiplex gene mutation, gene replacement and transcriptional control. As CRISPR/Cas9 is increasingly applied to plants, it is becoming clear that each component of the system can be modified to improve editing results. This review aims to highlight common considerations and options when conducting CRISPR/Cas9 experiments.
成簇规律间隔短回文重复序列（CRISPR）/CRISPR 相关核酸酶 9（Cas9）系统是一种基因组编辑技术，凭借其高效性和特异性改变了植物生物学领域。该系统已迅速发展出多种不同的应用，包括多基因突变、基因替换和转录控制。随着 CRISPR/Cas9 在植物中的应用越来越广泛，人们逐渐认识到可以对该系统的每个组成部分进行修改，以提高编辑效果。本综述旨在突出在进行 CRISPR/Cas9 实验时的常见考虑因素和选择。
