Payne Courtney, Sluiter Justin, Wolfrum Ed
The National Renewable Energy Laboratory, Golden, CO, USA.
Methods Mol Biol. 2019;1931:257-267. doi: 10.1007/978-1-4939-9039-9_18.
The publicly available NREL suite of laboratory analytical procedures (LAPs) provides researchers with the analytical tools to determine the composition of woody and herbaceous feedstocks. Feedstock characterization includes several steps: sample preparation, starch determination, moisture analysis, ashing, extraction, and hydrolysis. These steps provide information on specific compounds and classes of compounds such as ash, protein, moisture, extractives, sucrose, starch, glucan, xylan, galactan, arabinan, acetate and lignin. Here, we describe the use of these procedures to characterize sorghum [Sorghum bicolor (L.) Moench] with specific consideration of four main sorghum types: grain, forage, sweet, and biomass, which can vary significantly in composition. Special attention is paid to the extraction and the differentiation of glucose from starch and glucan from cellulose in the hybrids with significant amounts of nonstructural carbohydrates.
美国国家可再生能源实验室（NREL）公开的实验室分析程序（LAPs）套件为研究人员提供了用于确定木质和草本原料成分的分析工具。原料特性分析包括几个步骤：样品制备、淀粉测定、水分分析、灰化、提取和水解。这些步骤提供了有关特定化合物和化合物类别的信息，如灰分、蛋白质、水分、提取物、蔗糖、淀粉、葡聚糖、木聚糖、半乳聚糖、阿拉伯聚糖、乙酸盐和木质素。在此，我们描述了使用这些程序对高粱[Sorghum bicolor (L.) Moench]进行特性分析，并特别考虑了四种主要高粱类型：谷物型、饲料型、甜高粱型和生物质型，它们的成分可能有很大差异。对于含有大量非结构性碳水化合物的杂交品种，要特别注意从淀粉中提取葡萄糖以及从纤维素中区分葡聚糖。
