Faculty of Chemistry, Shahid Beheshti University, Evin, Tehran, Iran.
Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Electrophoresis. 2019 Apr;40(7):1074-1081. doi: 10.1002/elps.201800481. Epub 2019 Jan 30.
An electromembrane extraction followed by HPLC-UV technique was developed and validated for quantification of leuprolide and triptorelin in rabbit plasma. The influencing parameters on the extraction efficiency were optimized using experimental design methodology. The optimized conditions were found to be; supported liquid membrane: a mixture of 1-octanol and 2-ethyl hexanol (1:1) containing 10% v/v di(2-ethylhexyl) phosphate, applied voltage: 5 V, extraction time: 5 min, pH of the donor phase: 4.5 and pH of the acceptor phase: 1.0. The optimized method was validated for linearity, intraday and interday precision, and accuracy in rabbit plasma. The range of quantification for both peptides was 0.5-1000 ng/mL with regression coefficients higher than 0.994. Relative recoveries of leuprolide and triptorelin were found to be 80.3 and 75.5%, respectively. Limits of quantification and detection for both peptides were found to be 0.5 and 0.15 ng/mL, respectively. The validated method was successfully applied to pharmacokinetic study of the 1-month depot formulations of each peptide after subcutaneous administration to rabbits.
采用电渗析萃取结合高效液相色谱-紫外检测法，建立并验证了测定兔血浆中亮丙瑞林和曲普瑞林的方法。采用实验设计方法优化了萃取效率的影响参数。优化条件为：支撑液膜：1-辛醇和 2-乙基己醇（1:1）混合液，内含 10%v/v 二（2-乙基己基）磷酸，施加电压：5V，萃取时间：5min，供相 pH：4.5，受相 pH：1.0。该方法在兔血浆中进行了线性、日内和日间精密度及准确度验证。两种肽的定量范围均为 0.5-1000ng/mL，相关系数均高于 0.994。亮丙瑞林和曲普瑞林的相对回收率分别为 80.3%和 75.5%。两种肽的定量下限和检测下限分别为 0.5ng/mL 和 0.15ng/mL。该方法成功应用于兔皮下给予两种肽的 1 个月缓释制剂后的药代动力学研究。
