从哥斯达黎加消化不良患者中分离的幽门螺杆菌毒力因子基因分型。

Virulence factor genotyping of Helicobacter pylori isolated from Costa Rican dyspeptic patients.

机构信息

Institute for Health Research (INISA), University of Costa Rica, San Pedro, 11501, San José, Costa Rica; School of Medicine, University of Costa Rica, San Pedro, 11501, San José, Costa Rica.

Institute for Health Research (INISA), University of Costa Rica, San Pedro, 11501, San José, Costa Rica.

出版信息

Microb Pathog. 2019 Mar;128:276-280. doi: 10.1016/j.micpath.2019.01.018. Epub 2019 Jan 14.

DOI:10.1016/j.micpath.2019.01.018

PMID:30654009

Abstract

BACKGROUND AND AIMS

Costa Rica is one of the countries with the highest incidence and mortality rates for gastric cancer. Helicobacter pylori infection rates are high in the whole country. We have previously shown that H. pylori CagA+ is significantly associated with atrophic gastritis (AG) of the antrum in a dyspeptic population. The aim of this work is to determine if other H. pylori virulence factors (vacA, dupA, oipA, iceA and babA2) are associated with atrophic gastritis (AG) or duodenal ulcer (DU).

METHODS

The presence of virulence genes in Costa Rican H. pylori isolates was analyzed by PCR in 151 cultured strains from patients with dyspeptic symptoms. Endoscopic and histopathological diagnoses were available. Odds-ratio and 95% confidence intervals for AG patients vs. non-atrophic gastritis (NAG) or DU patients vs. no duodenal ulcer (NDU) patients were calculated.

RESULTS

Amongst the studied isolates, 82% had the cagA, 76.2% had the vacA s1m1, 97.0% had the oipA, 21.0% had the icea1, 79.0% had the iceA2, 44.0% had the babA2 and 76.0% the dupA genotypes. Infection with H pylori cagA, dupA, oipA, iceA, babA2, and vacA s1m1 genotypes was not associated with AG risk. The frequency of the dupA gene was 78.7 and 60.9% in isolates from patients with NDU and DU, respectively, and its presence was significantly associated with decreased risk of duodenal ulcer [odds-ratio: 0.33, p = 0.024, confidence interval 95% (0.11-0.85)].

CONCLUSION

H. pylori dupA genotype is inversely associated with DU risk in this population.

摘要

背景与目的

哥斯达黎加是胃癌发病率和死亡率最高的国家之一。全国幽门螺杆菌感染率很高。我们之前的研究表明，在消化不良人群中，幽门螺杆菌 CagA+与胃窦萎缩性胃炎（AG）显著相关。本研究旨在确定其他幽门螺杆菌毒力因子（vacA、dupA、oipA、iceA 和 babA2）是否与萎缩性胃炎（AG）或十二指肠溃疡（DU）相关。

方法

通过聚合酶链反应（PCR）分析了 151 株来自消化不良症状患者的培养菌株中幽门螺杆菌的毒力基因。可获得内镜和组织病理学诊断。计算了 AG 患者与非萎缩性胃炎（NAG）或 DU 患者与无十二指肠溃疡（NDU）患者的比值比（OR）和 95%置信区间（CI）。

结果

在所研究的分离株中，82%携带 cagA，76.2%携带 vacA s1m1，97.0%携带 oipA，21.0%携带 icea1，79.0%携带 iceA2，44.0%携带 babA2，76.0%携带 dupA 基因型。幽门螺杆菌 cagA、dupA、oipA、iceA、babA2 和 vacA s1m1 基因型感染与 AG 风险无关。NDU 和 DU 患者分离株中 dupA 基因的频率分别为 78.7%和 60.9%，其存在与十二指肠溃疡风险降低显著相关[比值比：0.33，p=0.024，95%置信区间（0.11-0.85）]。